Neuroserpin Recombinant antibody

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  WB analysis using 85923-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85923-1-RR (SERPINI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85923-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85923-1-RR (Neuroserpin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85923-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85923-1-RR (SERPINI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 85923-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85923-1-RR (Neuroserpin antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Neuroserpin Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Cat No. 85923-1-RR

Clone No.250112E7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, pig

Applications

WB, IHC, ELISA

Synonyms

Serpin I1, Peptidase inhibitor 12, PI 12, PI12, PI-12

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Tested Applications

• Positive WB detected in: fetal human brain tissue, mouse brain tissue, pig brain tissue, mouse cerebellum tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85923-1-RR targets Neuroserpin in WB, IHC, ELISA applications and shows reactivity with human, mouse, pig samples.

• Tested Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Neuroserpin fusion protein Eg2819
• Full Name: serpin peptidase inhibitor, clade I (neuroserpin), member 1
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: NM_005025.4
• Gene Symbol: Neuroserpin
• Gene ID (NCBI): 5274
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q99574
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neuroserpin is a member of the serpin family of serine protease inhibitors predominantly expressed in the nervous system, such as s the cerebral cortex, hippocampus and the spinal cord.Neuroserpin exerts protective effects in neurons against excitotoxicity both in vitro and in vivo. Its proteolytic inhibitory activity is shown to protect neurons against damage caused by plasmin activation. Mice overexpressing neuroserpin exhibited loss of tPA activity in the brain. Overexpression of neuroserpin in primary neurons leads to increased dendritic arborization and altered dendritic spine shape.