NeutraControl IL-10 Monoclonal antibody

NeutraControl IL-10 Monoclonal Antibody for Non-Neutralization, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

Non-Neutralization, ELISA

Conjugate

Unconjugated

CloneNo.

6C6E6

Cat no : 69518-1-Ig

Synonyms

CSIF, IL 10, IL10, IL-10, IL10A, interleukin 10, TGIF



Product Information

69518-1-Ig targets IL-10 in Non-Neutralization, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen human Humankine IL-10 protein HZ-1145
Full Name interleukin 10
Gene symbol IL10
Gene ID (NCBI) 3586
Conjugate Unconjugated
Form Lyophilized Powder
Purification Method Protein G purification
Storage Buffer Sterile PBS.
Endotoxin<0.1 EU/μg
ReconstitutionThis product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
Stability and StorageLyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Background Information

The human IL10 gene is 4.7 kb in size and is located on the long arm of chromosome 1. It contains five exons. Biologically functional IL-10 exists in the form of a 36 kDa homodimer composed of two non-covalently bonded mono-mers each with 160 amino acids length. IL-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

This antibody is a neutralizing control antibody for IL-10, the immunogen is the same as Neutrakine 69018-1-Ig but could not neutralize human IL-10.