NeutraControl IL-10 Monoclonal antibody

NeutraControl IL-10 Monoclonal Antibody for Non-Neutralization, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, Non-Neutralization, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

6C6E6

Cat no : 69518-1-Ig

Synonyms

CSIF, IL 10, IL10, IL-10, IL10A, interleukin 10, TGIF

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Validation Data Gallery

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  Non-Neutralization experiment of MC/9 cells using 69518-1-Ig

  Recombinant human IL-10 (Cat.NO. HZ-1145) stimulates proliferation of MC/9 cells (mouse mast cell line) in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human IL-10 (10 ng/mL) is neutralized by mouse anti-human IL-10 monoclonal antibody 69018-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 50-200 ng/mL. The NeutraControl mouse anti-human IL-10 monoclonal antibody 69518-1-Ig could recognize human IL-10 but could not neutralize human IL-10 in the range of 7.8125-2000ng/mL(green curve, refer to top X-right Y axis).

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  ELISA experiment of Recombinant protein using 69518-1-Ig

  Indirect ELISA was carried out by coating recombinant Human IL-10 (Cat.NO. HZ-1145) at 70 ng/well followed by blocking and adding serial diluted IL-10 antibody 69018-1-Ig and 69518-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.

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Published Applications

• WB: See 1 publications below

Product Information

69518-1-Ig targets IL-10 in WB, Non-Neutralization, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: human Humankine IL-10 protein HZ-1145
• Full Name: interleukin 10
• Gene Symbol: IL10
• Gene ID (NCBI): 3586
• RRID: AB_2918852
• Conjugate: Unconjugated
• Form: Lyophilized Powder
• Purification Method: Protein G purification
• Storage Buffer: Sterile PBS.
• Endotoxin: <0.1 EU/μg
• Reconstitution: This product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
• Stability and Storage: Lyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Background Information

The human IL10 gene is 4.7 kb in size and is located on the long arm of chromosome 1. It contains five exons. Biologically functional IL-10 exists in the form of a 36 kDa homodimer composed of two non-covalently bonded mono-mers each with 160 amino acids length. IL-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

This antibody is a neutralizing control antibody for IL-10, the immunogen is the same as Neutrakine 69018-1-Ig but could not neutralize human IL-10.

Publications

Species	Application	Title
mouse	WB	Cell Commun Signal; Schistosome egg-derived extracellular vesicles deliver Sja-miR-71a inhibits host macrophage and neutrophil extracellular traps via targeting Sema4D; Authors - Yao Liao

Related Products

NeutraKine® IL-10 Monoclonal antibody

69018-1-Ig