NeutraControl IL-10 Monoclonal antibody, PBS Only

• 

  Non-Neutralization experiment of MC/9 cells using 69518-1-Ig (same clone as 69518-1-PBS)

  Recombinant human IL-10 (Cat.NO. HZ-1145) stimulates proliferation of MC/9 cells (mouse mast cell line) in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human IL-10 (10 ng/mL) is neutralized by mouse anti-human IL-10 monoclonal antibody 69018-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 50-200 ng/mL. The NeutraControl mouse anti-human IL-10 monoclonal antibody 69518-1-Ig could recognize human IL-10 but could not neutralize human IL-10 in the range of 7.8125-2000ng/mL(green curve, refer to top X-right Y axis). This data was developed using the same antibody clone with 69518-1-PBS in a different storage buffer formulation.

• 

  ELISA experiment of Recombinant protein using 69518-1-Ig (same clone as 69518-1-PBS)

  Indirect ELISA was carried out by coating recombinant Human IL-10 (Cat.NO. HZ-1145) at 70 ng/well followed by blocking and adding serial diluted IL-10 antibody 69018-1-Ig and 69518-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm. This data was developed using the same antibody clone with 69518-1-PBS in a different storage buffer formulation.

NeutraControl IL-10 Monoclonal Antibody for ELISA, Non-Neutralization

Cat No. 69518-1-PBS

Clone No.6C6E6

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Synonyms

IL-10, IL-10,IL10, 6C6E6, CSIF, IL 10

---

69518-1-Ig

Product Information

69518-1-PBS targets IL-10 in applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: human Humankine IL-10 protein HZ-1145
• Full Name: interleukin 10
• Gene Symbol: IL-10
• Gene ID (NCBI): 3586
• Conjugate: Unconjugated
• Purification Method: Protein G purification
• Storage Buffer: PBS Only, pH 7.4.
• Storage Conditions: Store at -80°C.

Background Information

The human IL10 gene is 4.7 kb in size and is located on the long arm of chromosome 1. It contains five exons. Biologically functional IL-10 exists in the form of a 36 kDa homodimer composed of two non-covalently bonded mono-mers each with 160 amino acids length. IL-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

This antibody is a neutralizing control antibody for IL-10, the immunogen is the same as Neutrakine 69018-1-Ig but could not neutralize human IL-10.