NeutraControl PDGF-BB Monoclonal antibody, PBS Only

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• NeutraControl PDGF-BB Monoclonal antibody, PBS Only (69520-1-PBS)

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  Non-Neutralization experiment of BALB/3T3 cells using 69520-1-Ig (same clone as 69520-1-PBS)

  Recombinant human PDGF bb (Cat.NO. HZ-1308) stimulates the proliferation of BALB/3T3 cells (mouse fibroblast cell) in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human PDGF bb (20 ng/mL HZ-1308) is neutralized by mouse anti-human PDGF bb monoclonal antibody 69020-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 20-50 μg/mL. The NeutraControl mouse anti-human PDGF bb monoclonal antibody 69520-1-Ig could recognize human PDGF bb, but could not neutralize human PDGF bb at the range of 3.125-200 μg/mL(green curve, refer to top X-right Y axis). This data was developed using the same antibody clone with 69520-1-PBS in a different storage buffer formulation.

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  ELISA experiment of Recombinant protein using 69520-1-Ig (same clone as 69520-1-PBS)

  Indirect ELISA was carried out by coating recombinant Human PDGF bb (Cat.NO. HZ-1308) at 70 ng/well followed by blocking and adding serial diluted PDGF bb antibody 69020-1-Ig and 69520-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm. This data was developed using the same antibody clone with 69520-1-PBS in a different storage buffer formulation.

NeutraControl PDGF-BB Monoclonal Antibody for ELISA, Non-Neutralization

Cat No. 69520-1-PBS

Clone No.4E2C7

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

ELISA, Non-Neutralization

Synonyms

PDGFB, PDGFbb, Becaplermin, PDGF 2, PDGF subunit B

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Product Information

69520-1-PBS targets PDGF-BB in ELISA, Non-Neutralization applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo:

  Product name: HumanKine® recombinant human PDGFbb protein

  Source: -derived,

  Tag:

  Domain: of

  Sequence:

• Full Name: platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog)
• Gene Symbol: PDGFB
• Gene ID (NCBI): 5155
• Conjugate: Unconjugated
• Purification Method: Protein A purification
• UNIPROT ID: P01127
• Storage Buffer: PBS only, pH 7.3.
• Endotoxin: <0.1 EU/μg
• Storage Conditions: Store at -80°C.

Background Information

Platelet-derived growth factor (PDGF) belongs to the family of growth factors consisting of four secreted extracellular ligands including PDGF-A, -B, -C and -D. These isoforms, PDGF-AA, PDGF-AB, PDGF-BB, PDGF-CC and PDGF-DD, act via two receptor tyrosine kinases, PDGF receptors alpha and beta. PDGF isoforms stimulate growth, survival and motility of mesenchymal cells and certain other cell type. They have important functions during embryonal development and in the control of tissue homeostasis in the adult. PDGF-BB is one of the most recently studied isoforms and it plays an important role in skeletal development. PDGF-BB stimulates cell-mediated collagen gel contraction. PDGF-BB modulates endothelial proliferation and angiogenesis in vitro via PDGF beta-receptors.

This antibody is a neutralizing control antibody for PDGF-BB, the immunogen is the same as Neutrakine 69020-1-Ig but could not neutralize human PDGF-BB.