NeutraKine® BMP-7 Monoclonal antibody, PBS Only

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• NeutraKine® BMP-7 Monoclonal antibody, PBS Only (69011-1-PBS)

NeutraKine® BMP-7 Monoclonal Antibody for FC (Intra), Neutralization, Indirect ELISA

Cat No. 69011-1-PBS

Clone No.3E9G7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

FC (Intra), Neutralization, Indirect ELISA

Synonyms

BMP7, 3E9G7, BMP 7, BMP-7, Bone morphogenetic protein 7

• 

  Neutralization experiment of NeutraKine® BMP-7 using 69011-1-Ig (same clone as 69011-1-PBS)

  Recombinant human BMP-7 (Cat.NO. HZ-1229) induces alkaline phosphatase production in the ATDC-5 cell line (using pNPP as chromogenic substrate for detection) in a dose dependent manner (blue curve, bottom X-left Y). The activity of human BMP-7 (200 ng/mL HZ-1229) is neutralized by mouse anti-human BMP-7 antibody 69011-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 1-2 μg/mL. This data was developed using the same antibody clone with 69011-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of HEK-293 using 69011-1-Ig (same clone as 69011-1-PBS)

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human NeutraKine® BMP-7 (69011-1-Ig, Clone:3E9G7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 69011-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

69011-1-PBS targets NeutraKine® BMP-7 in FC (Intra), Neutralization, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo:

  Product name: HumanKine® recombinant human BMP-7 protein

  Source: -derived,

  Tag:

  Domain: of

  Sequence:

• Full Name: bone morphogenetic protein 7
• Gene Symbol: BMP7
• Gene ID (NCBI): 655
• Conjugate: Unconjugated
• Purification Method: Protein G purification
• UNIPROT ID: P18075
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site.

BMP7, also known as osteogenic protein-1 or OP-1, plays a key role in the transformation of mesenchymal cells into bone and cartilage. BMP7 may be involved in bone homeostasis (PMID: 15621726). It is expressed in the brain, kidneys and bladder. BMP7 is also present in cancers, including breast, prostate, and colon cancers, in which it is implicated in regulating cancer cell proliferation (PMID: 16419056, PMID: 15531927). Overexpression of BMP7 mRNA in colorectal cancer patients was significantly associated with poor prognosis and low overall survival (PMID: 18259822). Recent studies suggest that high-expression level of BMP7 serves as a biomarker for poor prognosis for HCC (PMID: 23179403).

This antibody can be used to neutralize the bioactivity of BMP-7.