NeutraKine®FGF basic Monoclonal antibody, PBS Only (Detector)

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  Neutralization experiment of NeutraKine®FGF basic using 69024-1-Ig (same clone as 69024-1-PBS)

  Recombinant human FGF Basic (Cat.NO. HZ-1285) stimulates the proliferation of BALB/3T3 cells (mouse fibroblast cell) at low FBS condition in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human FGF Basic (0.1 ng/mL HZ-1285) is neutralized by mouse anti-human FGF Basic monoclonal antibody 69024-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 1-10 μg/mL. Mouse IgG1 Isotype Control monoclonal antibody 66360-1-Ig was served as control (green curve, refer to top X-right Y axis). This data was developed using the same antibody clone with 69024-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50262-2

  Cytometric bead array standard curve of MP50262-2, FGF2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68870-2-PBS. Detection antibody: 69024-1-PBS. Standard:HZ-1285. Range: 0.391-100 ng/mL.

NeutraKine®FGF basic Monoclonal Antibody for Neutralization, Cytometric bead array, Indirect ELISA

Cat No. 69024-1-PBS

Clone No.3C9C3

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Neutralization, Cytometric bead array, Indirect ELISA

Synonyms

FGF2, FGF-2, 3C9C3, FGF 2, FGF B

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Product Information

69024-1-PBS targets NeutraKine®FGF basic as part of a matched antibody pair:

MP50262-2: 68870-2-PBS capture and 69024-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: human HumankineFGF basic protein HZ-1285
• Full Name: fibroblast growth factor 2 (basic)
• Gene Symbol: FGF2
• Gene ID (NCBI): 2247
• RRID: AB_2918844
• Conjugate: Unconjugated
• Purification Method: Protein G purification
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Fibroblast growth factor-2 (FGF-2), also referred to as basic FGF, belongs to a family of growth factors that stimulate proliferation of cells of mesenchymal, epithelial and neuroectodermal origin. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in at least five different isoforms with distinct properties.

This antibody is used to neutralize the bioactivity of FGFbasic-TS.