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CoraLite®555-conjugated NeutraKine® IFN Gamma Monoclonal antibody

NeutraKine® IFN Gamma Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

1E10G7

Cat no : CL555-69007

Synonyms

IFG, IFI, IFN gamma, IFNG, Immune interferon, Interferon gamma, interferon, gamma

Validation Data Gallery

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  • Flow cytometry (FC) experiment of Jurkat cells using CoraLite®555-conjugated NeutraKine® IFN Gamma Mono (CL555-69007)

    FC experiment of Jurkat using CL555-69007

    1X10^6 PMA and ionomycin treated Jurkat cells were intracellularly stained with 0.25 ug CoraLite®555 Anti-Human NeutraKine® IFN Gamma (CL555-69007, Clone:1E10G7), and 0.25 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inPMA and ionomycin treated Jurkat cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-69007 targets NeutraKine® IFN Gamma in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NeutraKine® IFN Gamma fusion protein HZ-1301
Full Name IFN gamma
Gene Symbol IFNG
Gene ID (NCBI) 3458
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interferon gamma (IFNG) is a soluble cytokine that is the only member of the type II class of interferons. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays crucial roles in pathogen clearance. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB). Mutations in this gene are associated with aplastic anemia.

This antibody can be used to neutralize the bioactivity of Interferon gamma.

Protocols

Product Specific Protocols
FC protocol for CL555 NeutraKine® IFN Gamma antibody CL555-69007Download protocol
Standard Protocols
Click here to view our Standard Protocols