Nicastrin Recombinant monoclonal antibody, PBS Only

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  WB analysis using 87471-1-RR (same clone as 87471-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87471-1-RR (Nicastrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87471-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 87471-1-RR (same clone as 87471-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87471-1-RR (Nicastrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87471-1-PBS in a different storage buffer formulation.

• 

  IF Staining of C6 using 87471-1-RR (same clone as 87471-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed C6 cells using Nicastrin antibody (87471-1-RR, Clone: 252886F8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87471-1-PBS in a different storage buffer formulation.

• 

  Affinity of 87471-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87471-RR against Human Nicastrin were performed. The affinity constant is 1.23 nM.

Nicastrin Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 87471-1-PBS

Clone No.252886F8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

KIAA0253, NCSTN, UNQ1874/PRO4317

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Product Information

87471-1-PBS targets Nicastrin in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag5137

  Product name: Recombinant human Nicastrin protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 338-689 aa of BC047621

  Sequence: NVDSFVELGQVALRTSLELWMHTDPVSQKNESVRNQVEDLLATLEKSGAGVPAVILRRPNQSQPLPPSSLQRFLRARNISGVVLADHSGAFHNKYYQSIYDTAENINVSYPEWLSPEEDLNFVTDTAKALADVATVLGRALYELAGGTNFSDTVQADPQTVTRLLYGFLIKANNSWFQSILRQDLRSYLGDGPLQHYIAVSSPTNTTYVVQYALANLTGTVVNLTREQCQDPSKVPSENKDLYEYSWVQGPLHSNETDRLPRCVRSTARLARALSPAFELSQWSSTEYSTWTESRWKDIHARIFLIASKELELITLTVGFGILIFSLIVTYCINAKADVLFIAPREPGAVSY

• Full Name: nicastrin
• Calculated Molecular Weight: 709 aa, 78.4 kDa
• Observed Molecular Weight: 110 kDa, 130 kDa
• GenBank Accession Number: BC047621
• Gene Symbol: Nicastrin
• Gene ID (NCBI): 23385
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q92542
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Nicastrin (NCT) is a type I transmembrane glycoprotein, which forms high molecular weight gamma-secretase complexes with presenilin 1 and presenilin 2 (PMID: 16096051). The gamma-secretase is an intramembrane protease that cleaves many type I membrane proteins, including amyloid precursor protein (APP) and Notch, generating peptide fragments that are important signaling components (PMID: 16096062). The proteolytic processing of APP results in the release of amyloid-βpeptide, a principal component of the plaques found in the brains of Alzheimer's disease patients (PMID: 12716934).