Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells, NCCIT cells, HT-29 cells |
| Positive IHC detected in | mouse brain tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MDCK cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67132-1-Ig targets Ninein in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, canine samples.
| Tested Reactivity | human, mouse, canine |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14652 Product name: Recombinant human Ninein protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1870-2020 aa of BC034708 Sequence: VRQLESNLLPKHQKHLNPSGTMNPTEQEKLSLKRECDQFQKEQSPANRKVSQMNSLEQELETIHLENEGLKKKQVKLDEQLMEMQHLRSTATPSPSPHAWDLQLLQQQACPMVPREQFLQLQRQLLQAERINQHLQEELENRTSETNTPQ Predict reactive species |
| Full Name | ninein (GSK3B interacting protein) |
| Calculated Molecular Weight | 2090 aa, 243 kDa |
| Observed Molecular Weight | 220 kDa |
| GenBank Accession Number | BC034708 |
| Gene Symbol | Ninein |
| Gene ID (NCBI) | 51199 |
| RRID | AB_2882431 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q8N4C6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Ninein is a large coiled-coil protein that associates with the subdistal appendages of the mother centriole and the minus-ends of both centrioles. Ninein interacts with γ-tubulin and microtubule nucleation and anchoring at the centrosomes.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Ninein antibody 67132-1-Ig | Download protocol |
| IHC protocol for Ninein antibody 67132-1-Ig | Download protocol |
| WB protocol for Ninein antibody 67132-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Ninein Monoclonal antibody (1B10A8), catalog number 67132-1-Ig, has 3 total citations. These appear in Developmental Cell, Journal of Cell Science, and eLife. The research fields span centrosome proteomic profiling, cilia function and dynein-2 assembly, and ciliogenesis/cerebellar development in the context of trisomy 21. Applications cited include Western blot and immunofluorescence, with human as the reactive species.
| Species | Application | Title |
|---|---|---|
Dev Cell Proteomic profiling of centrosomes across multiple mammalian cell and tissue types by an affinity capture method
| ||
Elife Trisomy 21 induces pericentrosomal crowding delaying primary ciliogenesis and mouse cerebellar development |
Reviews
What customers say
Both reviews rate the antibody 5 stars. One user found it effective at 1:300 for IF and 1:500 for WB, noting clear staining of the ninein pool at the centrosome in HAP1, H9, RPE1, and HEK293T cells using methanol fixation. The other user successfully used it at 1:1000 for Western Blot to visualize ninein protein in Hap1 control and Ninein knockout cells. Overall, customers report strong performance in both WB and IF applications with good specificity for ninein.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Elisa (Verified Customer) (07-12-2021) | This antibody stains well ninein pool at the centrosome, in MetOH fixation conditions
|
Sarah (Verified Customer) (07-06-2020) | Antibody used at 1:1000 to visualise ninein protein (from 20ug cell lysate) in Hap1 control and Ninein KO cells
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