Ninein Monoclonal antibody, PBS Only

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  WB analysis using 67132-1-Ig (same clone as 67132-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67132-1-Ig (Ninein antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67132-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67132-1-Ig (same clone as 67132-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67132-1-Ig (Ninein antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67132-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67132-1-Ig (same clone as 67132-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67132-1-Ig (Ninein antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67132-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 67132-1-Ig (same clone as 67132-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67132-1-Ig (Ninein antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67132-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 67132-1-Ig (same clone as 67132-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67132-1-Ig (Ninein antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67132-1-PBS in a different storage buffer formulation.

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  IF Staining of MDCK using 67132-1-Ig (same clone as 67132-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using 67132-1-Ig (Ninein antibody) at dilution of 1:500 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67132-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

Ninein Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 67132-1-PBS

Clone No.1B10A8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, canine

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

NIN, 1B10A8, Glycogen synthase kinase 3 beta-interacting protein, GSK3B interacting protein, GSK3B-interacting protein

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Product Information

67132-1-PBS targets Ninein in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, canine samples.

• Tested Reactivity: human, mouse, canine
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14652

  Product name: Recombinant human Ninein protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1870-2020 aa of BC034708

  Sequence: VRQLESNLLPKHQKHLNPSGTMNPTEQEKLSLKRECDQFQKEQSPANRKVSQMNSLEQELETIHLENEGLKKKQVKLDEQLMEMQHLRSTATPSPSPHAWDLQLLQQQACPMVPREQFLQLQRQLLQAERINQHLQEELENRTSETNTPQ

• Full Name: ninein (GSK3B interacting protein)
• Calculated Molecular Weight: 2090 aa, 243 kDa
• Observed Molecular Weight: 220 kDa
• GenBank Accession Number: BC034708
• Gene Symbol: Ninein
• Gene ID (NCBI): 51199
• RRID: AB_2882431
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q8N4C6
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Ninein is a large coiled-coil protein that associates with the subdistal appendages of the mother centriole and the minus-ends of both centrioles. Ninein interacts with γ-tubulin and microtubule nucleation and anchoring at the centrosomes.