OAT Recombinant monoclonal antibody

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• OAT Recombinant monoclonal antibody (85218-2-RR)

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  WB analysis using 85218-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85218-2-RR (OAT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 85218-2-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using OAT antibody (85218-2-RR, Clone: 242843D8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

OAT Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 85218-2-RR

Clone No.242843D8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

242843D8, EC:2.6.1.13, GACR, HOGA, OATASE

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Tested Applications

• Positive WB detected in: K-562 cells, HCT 116 cells, HepG2 cells, mouse colon tissue, mouse lung tissue, rat colon tissue, rat lung tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85218-2-RR targets OAT in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag9981

  Product name: Recombinant human OAT protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-439 aa of BC000964

  Sequence: MFSKLAHLQRFAVLSRGVHSSVASATSVATKKTVQGPPTSDDIFEREYKYGAHNYHPLPVALERGKGIYLWDVEGRKYFDFLSSYSAVNQGHCHPKIVNALKSQVDKLTLTSRAFYNNVLGEYEEYITKLFNYHKVLPMNTGVEAGETACKLARKWGYTVKGIQKYKAKIVFAAGNFWGRTLSAISSSTDPTSYDGFGPFMPGFDIIPYNDLPALERALQDPNVAAFMVEPIQGEAGVVVPDPGYLMGVRELCTRHQVLFIADEIQTGLARTGRWLAVDYENVRPDIVLLGKALSGGLYPVSAVLCDDDIMLTIKPGEHGSTYGGNPLGCRVAIAALEVLEEENLAENADKLGIILRNELMKLPSDVVTAVRGKGLLNAIVIKETKDWDAWKVCLRLRDNGLLAKPTHGDIIRFAPPLVIKEDELRESIEIINKTILSF

• Full Name: ornithine aminotransferase (gyrate atrophy)
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC000964
• Gene Symbol: OAT
• Gene ID (NCBI): 4942
• RRID: AB_3744110
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P04181
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ornithine-delta-aminotransferase (OAT) (EC 2.6.1.13) is a pyridoxal-5' phosphate dependent mitochondrial matrix enzyme. It controls the L-ornithine (Orn) level in tissues by catalysing the transfer of the delta-amino group of Orn to 2-oxoglutarate. OAT an enzyme mainly expressed in liver. (PMID: 11465067, PMID: 36647689)