OBFC2B Polyclonal antibody

• Featured Product
• KD/KO Validated

OBFC2B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 14809-1-AP

Synonyms

Sensor of single-strand DNA complex subunit B1, Oligonucleotide/oligosaccharide-binding fold-containing protein 2B, Nucleic acid-binding protein 2, NABP2, LP3587

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Validation Data Gallery

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  WB analysis using 14809-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14809-1-AP (OBFC2B antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 14809-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14809-1-AP (OBFC2B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14809-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14809-1-AP (OBFC2B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14809-1-AP

  IP result of anti-OBFC2B (IP:14809-1-AP, 4ug; Detection:14809-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IP experiment of HeLa using 14809-1-AP

  IP result of anti-OBFC2B (IP:14809-1-AP, 4ug; Detection:14809-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IHC staining of human ovary tumor using 14809-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14809-1-AP (OBFC2B Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas using 14809-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14809-1-AP (OBFC2B Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 14809-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14809-1-AP (OBFC2B Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary tumor using 14809-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14809-1-AP (OBFC2B Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 14809-1-AP

  Immunofluorescent analysis of Hela cells, using OBFC2B antibody 14809-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HeLa using 14809-1-AP

  Immunofluorescent analysis of Hela cells, using OBFC2B antibody 14809-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HepG2 cells, NIH/3T3 cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human ovary tumor tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

14809-1-AP targets OBFC2B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OBFC2B fusion protein Ag6532
• Full Name: oligonucleotide/oligosaccharide-binding fold containing 2B
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 30-33 kDa
• GenBank Accession Number: BC001079
• Gene Symbol: OBFC2B
• Gene ID (NCBI): 79035
• RRID: AB_2251673
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OBFC2B, also named as NABP2 or SSB1, is a 211 amino acid protein, which belongs to the SOSS-B family. OBFC2B localizes to nuclear foci following DNA damage. OBFC2B is a component of the SOSS complex, a multiprotein complex that functions downstream of the MRN complex to promote DNA repair and G2/M checkpoint. In the SOSS complex, OBFC2B acts as a sensor of single-stranded DNA that binds to single-stranded DNA, in particular to polypyrimidines. The calculated molecular weight of OBFC2B is 22 kDa, but modified OBFC2B is about 32 kDa.

Publications

Species	Application	Title
human	WB	Cell Res; The Integrator complex controls the termination of transcription at diverse classes of gene targets.; Authors - Jeffrey R Skaar ; KD Validated
human	WB	Cell Death Dis; Nitric oxide triggers the assembly of "type II" stress granules linked to decreased cell viability.; Authors - Anaïs Aulas
human	WB,IHC	J Inflamm Res; Comprehensive Analysis of NABP2 as a Prognostic Biomarker and Its Correlation with Immune Infiltration in Hepatocellular Carcinoma; Authors - Bowen Li