ODF1 Polyclonal antibody

ODF1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 24736-1-AP

Synonyms

HSPB10, ODF, ODF1, ODF2, ODF27, ODFP, ODFPG, ODFPGA, ODFPGB, Outer dense fiber protein 1, RT7, SODF

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Validation Data Gallery

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  WB analysis of rat testis using 24736-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 24736-1-AP (ODF1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 24736-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24736-1-AP (ODF1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 24736-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24736-1-AP (ODF1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 24736-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24736-1-AP (ODF1 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: rat testis tissue, mouse testis tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

The immunogen of 24736-1-AP is ODF1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, rat, mouse
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ODF1 fusion protein Ag20562
• Full Name: outer dense fiber of sperm tails 1
• Calculated Molecular Weight: 250 aa, 28 kDa
• Observed Molecular Weight: 28 kDa, 56 kDa
• GenBank Accession Number: BC104457
• Gene Symbol: ODF1
• Gene ID (NCBI): 4956
• RRID: AB_2879697
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ODF1, also named as Outer dense fiber protein 1, is a 250 amino acid protein, which contains the C-terminal contains many C-X-P repeats. ODF1 is a component of the outer dense fibers (ODF) of spermatozoa. ODF27 that suggested the possibility of inter- actions between ODF27 itself and/or between ODF27 and other sperm tail proteins.

Publications

Species	Application	Title
pig	WB	Front Cell Dev Biol; Comparative Proteomics and Phosphoproteomics Analysis Reveal the Possible Breed Difference in Yorkshire and Duroc Boar Spermatozoa.; Authors - Yongjie Xu
human	WB	Mol Genet Genomic Med; Novel mutations of PMFBP1 in a man with acephalic spermatozoa defects.; Authors - Hua Nie
mouse	WB	Cell Mol Life Sci; CCDC146 is required for sperm flagellum biogenesis and male fertility in mice; Authors - Yanjie Ma