OGT Recombinant monoclonal antibody, PBS Only

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  WB analysis using 83113-1-RR (same clone as 83113-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83113-1-RR (OGT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83113-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83113-1-RR (same clone as 83113-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OGT antibody (83113-1-RR, Clone: 230385D11 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83113-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83113-1-RR (same clone as 83113-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.2 ug OGT Recombinant antibody (83113-1-RR, Clone:230385D11)(red), or 0.2 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83113-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83113-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83113-1-RR against Human OGT were performed. The affinity constant is below 1 pM.

OGT Uni-rAb^® Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 83113-1-PBS

Clone No.230385D11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

O-linked N-acetylglucosamine transferase 110 kDa subunit, O-GlcNAc transferase subunit p110, O GLCNAC, HRNT1, EC:2.4.1.255

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Product Information

83113-1-PBS targets OGT in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag2160

  Product name: Recombinant human OGT protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-400 aa of BC014434

  Sequence: MASSVGNVADSTGLAELAHREYQAGDFEAAERHCMQLWRQEPDNTGVLLLLSSIHFQCRRLDRSAHFSTLAIKQNPLLAEAYSNLGNVYKERGQLQEAIEHYRHALRLKPDFIDGYINLAAALVAAGDMEGAVQAYVSALQYNPDLYCVRSDLGNLLKALGRLEEAKACYLKAIETQPNFAVAWSNLGCVFNAQGEIWLAIHHFEKAVTLDPNFLDAYINLGNVLKEARIFDRAVAAYLRALSLSPNHAVVHGNLACVYYEQGLIDLAIDTYRRAIELQPHFPDAYCNLANALKEKGSVAEAEDCYNTALRLCPTHADSLNNLANIKREQGNIEEAVRLYRKALEVFPEFAAAHSNLASVLQQQGKLQEALMHYKEAIRISPTFADAYSNMGNTLKEMQD

• Full Name: O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)
• Calculated Molecular Weight: 1046 aa, 117 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC014434
• Gene Symbol: OGT
• Gene ID (NCBI): 8473
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O15294
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

O-linked N-acetylglucosamine transferase (OGT) catalyzes the attachment of N-acetylglucosamine (GlcNAc) monosaccharides to the hydroxyl group of serine or threonine residues of numerous nuclear and cytoplasmic proteins and may play important roles in a large number of diverse intracellular processes ranging from translational control, transcription, transcriptional repression, INS resistance and regulation of the cell cycle. It exists as a heterotrimeric complex with two 110 kDa and one 70 kDa subunits. Recent studies have shown that O-GlcNAcylation plays essential roles in cancer formation and progression. O-GlcNAcylation as well as OGT expression was found to be significantly elevated in the cancer tissues.