OPA1 Monoclonal antibody

• Featured Product
• KD/KO Validated

OPA1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, pig, rat and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

19

CloneNo.

1B2D8

Cat no : 66583-1-Ig

Synonyms

KIAA0567, largeG, MGM1, NTG, OPA1, Optic atrophy protein 1

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Validation Data Gallery

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  WB analysis using 66583-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66583-1-Ig (OPA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66583-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66583-1-Ig (OPA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66583-1-Ig

  WB result of OPA1 antibody (66583-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-OPA1 transfected HEK-293 cells.

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  IHC staining of mouse brain using 66583-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66583-1-Ig (OPA1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 66583-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66583-1-Ig (OPA1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, pig brain tissue, HeLa cells, HepG2 cells, Y79 cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 19 publications below
• IF: See 3 publications below

Product Information

66583-1-Ig targets OPA1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, pig, rat samples.

• Tested Reactivity: Human, mouse, pig, rat
• Cited Reactivity: human, mouse, rat, fish
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: OPA1 fusion protein Ag26868
• Full Name: optic atrophy 1 (autosomal dominant)
• Calculated Molecular Weight: 960 aa, 112 kDa
• Observed Molecular Weight: 100 kDa and 80-90 kDa
• GenBank Accession Number: BC075805
• Gene Symbol: OPA1
• Gene ID (NCBI): 4976
• RRID: AB_2881943
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OPA1 is a nuclear-encoded mitochondrial protein with similarity to dynamin-related GTPases. OPA1 localizes to the inner mitochondrial membrane and helps regulate mitochondrial stability and energy output. This protein also sequesters cytochrome c. OPA1 is associated with the inner membrane and protects cells from apoptosis by regulating inner membrane dynamics. Mutation of OPA1 causes the disease dominant optic atrophy, a degeneration of the retinal ganglion cells. OPA1 undergoes complex posttranscriptional regulation and posttranslational proteolysis. OPA1 is regulated by proteolytic cleavage, which degrades long OPA1 isoforms into short isoforms. The gene OPA1 can be cleaved into some chains with MW 100 kDa and 80-90 kDa.

Publications

Species	Application	Title
mouse	WB,IF	J Agric Food Chem; Pyrroloquinoline Quinone Alleviates Mitochondria Damage in Radiation-Induced Lung Injury in a MOTS-c-Dependent Manner; Authors - Yanli Zhang
mouse	WB	J Endocrinol; ChREBP-β regulates thermogenesis in brown adipose tissue.; Authors - Chunchun Wei
fish	WB	J Physiol Sci; Cardioprotective responses to aerobic exercise-induced physiological hypertrophy in zebrafish heart; Authors - Zhanglin Chen
	WB	Behav Brain Res; Protective effect of exercise training against the progression of Alzheimer's disease in 3xTg-AD mice.; Authors - Donghyun Kim
human,mouse	WB	Biochim Biophys Acta Mol Basis Dis; SENP1 prevents high fat diet-induced non-alcoholic fatty liver diseases by regulating mitochondrial dynamics; Authors - Wenjing Zeng
mouse	WB	Biochim Biophys Acta Mol Basis Dis; Electroacupuncture pretreatment maintains mitochondrial quality control via HO-1/MIC60 signaling pathway to alleviate endotoxin-induced acute lung injury; Authors - Jia Shi