OPA1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

OPA1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, pig, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1B2D8

Cat no : 66583-1-PBS

Synonyms

FLJ12460, KIAA0567, largeG, MGM1, NTG, OPA1, Optic atrophy protein 1

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Validation Data Gallery

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  WB analysis using 66583-1-Ig (same clone as 66583-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66583-1-Ig (OPA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66583-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66583-1-Ig (same clone as 66583-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66583-1-Ig (OPA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66583-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66583-1-Ig (same clone as 66583-1-PBS)

  WB result of OPA1 antibody (66583-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-OPA1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66583-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66583-1-Ig (same clone as 66583-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66583-1-Ig (OPA1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66583-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66583-1-Ig (same clone as 66583-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66583-1-Ig (OPA1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66583-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66583-1-PBS targets OPA1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, pig, rat samples.

• Tested Reactivity: Human, mouse, pig, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: OPA1 fusion protein Ag26868
• Full Name: optic atrophy 1 (autosomal dominant)
• Calculated Molecular Weight: 960 aa, 112 kDa
• Observed Molecular Weight: 100 kDa and 80-90 kDa
• GenBank Accession Number: BC075805
• Gene Symbol: OPA1
• Gene ID (NCBI): 4976
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -20°C. Stable for one year after shipment.

Background Information

OPA1 is a nuclear-encoded mitochondrial protein with similarity to dynamin-related GTPases. OPA1 localizes to the inner mitochondrial membrane and helps regulate mitochondrial stability and energy output. This protein also sequesters cytochrome c. OPA1 is associated with the inner membrane and protects cells from apoptosis by regulating inner membrane dynamics. Mutation of OPA1 causes the disease dominant optic atrophy, a degeneration of the retinal ganglion cells. OPA1 undergoes complex posttranscriptional regulation and posttranslational proteolysis. OPA1 is regulated by proteolytic cleavage, which degrades long OPA1 isoforms into short isoforms. The gene OPA1 can be cleaved into some chains with MW 100 kDa and 80-90 kDa.