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  • KD/KO Validated

OPRL1 Polyclonal antibody

OPRL1 Polyclonal Antibody for WB, IP, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, FC, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12970-1-AP

Synonyms

Kappa type 3 opioid receptor, KOR 3, Nociceptin receptor, NOCIR, OOR, opiate receptor like 1, OPRL1, ORL1, Orphanin FQ receptor



Tested Applications

Positive WB detected inHeLa cells, SH-SY5Y cells
Positive IP detected inSH-SY5Y cells
Positive IHC detected inhuman gliomas tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12970-1-AP targets OPRL1 in WB, IP, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OPRL1 fusion protein Ag4079
Full Name opiate receptor-like 1
Calculated Molecular Weight 370 aa, 41 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC038433
Gene Symbol OPRL1
Gene ID (NCBI) 4987
RRIDAB_10638603
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The OPRL1 (opioid related nociceptin receptor 1) is the G-protein coupled receptor activated by the endogenou NC (neuropeptide nociceptin) (PMID:7566152). OPRL1 is widely expressed in the central nervous system, including the hippocampal dentate gyrus, cortical areas, striatum, thalamus, and hypothalamus (PMID:19501074). OPRL1 has been found to play an essential role in cognition, and to modulate inflammation and immune responses (PMID:30152845).

Protocols

Product Specific Protocols
WB protocol for OPRL1 antibody 12970-1-APDownload protocol
IHC protocol for OPRL1 antibody 12970-1-APDownload protocol
IP protocol for OPRL1 antibody 12970-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB,IHC

Int Immunopharmacol

PMID: 31865244

Authors - Neili Xu
humanWB

Cell Death Discov

The nociceptin receptor promotes autophagy through NF-kB signaling and is transcriptionally regulated by E2F1 in HCC.

Authors - Xiaoshuang Zhou
  • KD Validated
ratWB

Biomed Res Int

PMID: 25276817

Authors - Katarzyna Popiolek-Barczyk
rat

Biochim Biophys Acta

Pharmacological blockade of CXCR3 by (±)-NBI-74330 reduces neuropathic pain and enhances opioid effectiveness - Evidence from in vivo and in vitro studies.

Authors - Anna Piotrowska