Tested Applications
| Positive WB detected in | HeLa cells, SH-SY5Y cells |
| Positive IP detected in | SH-SY5Y cells |
| Positive IHC detected in | human gliomas tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12970-1-AP targets OPRL1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4079 Product name: Recombinant human OPRL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 71-370 aa of BC038433 Sequence: LVMYVILRHTKMKTATNIYIFNLALADTLVLLTLPFQGTDILLGFWPFGNALCKTVIAIDYYNMFTSTFTLTAMSVDRYVAICHPIRALDVRTSSKAQAVNVAIWALASVVGVPVAIMGSAQVEDEEIECLVEIPTPQDYWGPVFAICIFLFSFIVPVLVISVCYSLMIRRLRGVRLLSGSREKDRNLRRITRLVLVVVAVFVGCWTPVQVFVLAQGLGVQPSSETAVAILRFCTALGYVNSCLNPILYAFLDENFKACFRKFCCASALRRDVQVSDRVRSIAKDVALACKTSETVPRPA Predict reactive species |
| Full Name | opiate receptor-like 1 |
| Calculated Molecular Weight | 370 aa, 41 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC038433 |
| Gene Symbol | OPRL1 |
| Gene ID (NCBI) | 4987 |
| RRID | AB_10638603 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P41146 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The OPRL1 (opioid related nociceptin receptor 1) is the G-protein coupled receptor activated by the endogenou NC (neuropeptide nociceptin) (PMID:7566152). OPRL1 is widely expressed in the central nervous system, including the hippocampal dentate gyrus, cortical areas, striatum, thalamus, and hypothalamus (PMID:19501074). OPRL1 has been found to play an essential role in cognition, and to modulate inflammation and immune responses (PMID:30152845).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for OPRL1 antibody 12970-1-AP | Download protocol |
| IP protocol for OPRL1 antibody 12970-1-AP | Download protocol |
| WB protocol for OPRL1 antibody 12970-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
12970-1-AP (Anti-Nociceptin Receptor) has 8 total citations published in Cell Death Discovery, Environmental Pollution, European Journal of Pharmacology, International Immunopharmacology, Biochimica et Biophysica Acta, Biomedical Research International, Neuropeptides, and bioRxiv. Research fields span hepatocellular carcinoma autophagy signaling, neuroendocrine regulation of puberty, diabetic peripheral neuropathy, inflammatory arthritis, neuropathic pain mechanisms, and microglial state characterization.
| Species | Application | Title |
|---|---|---|
Cell Death Discov The nociceptin receptor promotes autophagy through NF-kB signaling and is transcriptionally regulated by E2F1 in HCC.
| ||
Environ Pollut Phenanthrene exposure disrupts puberty onset in SD rats by influencing hypothalamic neural networks | ||
Eur J Pharmacol UFP-101 alleviates diabetic peripheral neuropathy through membrane metallo-endopeptidase. | ||
Biochim Biophys Acta Pharmacological blockade of CXCR3 by (±)-NBI-74330 reduces neuropathic pain and enhances opioid effectiveness - Evidence from in vivo and in vitro studies. | ||
