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  • KD/KO Validated

OPRL1 Polyclonal antibody

OPRL1 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 12970-1-AP

Synonyms

Kappa type 3 opioid receptor, KOR 3, Nociceptin receptor, NOCIR, OOR, opiate receptor like 1, OPRL1, ORL1, Orphanin FQ receptor



Tested Applications

Positive WB detected inHeLa cells, SH-SY5Y cells
Positive IP detected inSH-SY5Y cells
Positive IHC detected inhuman gliomas tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

12970-1-AP targets OPRL1 in WB, IP, IHC, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OPRL1 fusion protein Ag4079
Full Name opiate receptor-like 1
Calculated molecular weight 370 aa, 41 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC038433
Gene symbol OPRL1
Gene ID (NCBI) 4987
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for OPRL1 antibody 12970-1-APDownload protocol
IHC protocol for OPRL1 antibody 12970-1-APDownload protocol
IP protocol for OPRL1 antibody 12970-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB,IHC

Int Immunopharmacol

PMID: 31865244

Authors - Neili Xu
humanWB

Cell Death Discov

The nociceptin receptor promotes autophagy through NF-kB signaling and is transcriptionally regulated by E2F1 in HCC.

Authors - Xiaoshuang Zhou
  • KD Validated
ratWB

Biomed Res Int

PMID: 25276817

Authors - Katarzyna Popiolek-Barczyk
rat

Biochim Biophys Acta

Pharmacological blockade of CXCR3 by (±)-NBI-74330 reduces neuropathic pain and enhances opioid effectiveness - Evidence from in vivo and in vitro studies.

Authors - Anna Piotrowska