OPRL1 Polyclonal antibody

• Featured Product
• KD/KO Validated

OPRL1 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, FC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 12970-1-AP

Synonyms

Kappa type 3 opioid receptor, KOR 3, Nociceptin receptor, NOCIR, OOR, opiate receptor like 1, OPRL1, ORL1, Orphanin FQ receptor

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Validation Data Gallery

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  WB analysis of HeLa using 12970-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12970-1-AP (OPRL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 12970-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 12970-1-AP (OPRL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of SH-SY5Y using 12970-1-AP

  IP result of anti-OPRL1 (IP:12970-1-AP, 4ug; Detection:12970-1-AP 1:500) with SH-SY5Y cells lysate 2000ug.

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  IHC staining of human gliomas using 12970-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 12970-1-AP (OPRL1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human gliomas using 12970-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 12970-1-AP (OPRL1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of mouse brain using 12970-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12970-1-AP (OPRL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of SH-SY5Y using 12970-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug OPRL1 antibody (12970-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Tested Applications

• Positive WB detected in: HeLa cells, SH-SY5Y cells
• Positive IP detected in: SH-SY5Y cells
• Positive IHC detected in: human gliomas tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

12970-1-AP targets OPRL1 in WB, IP, IHC, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OPRL1 fusion protein Ag4079
• Full Name: opiate receptor-like 1
• Calculated Molecular Weight: 370 aa, 41 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC038433
• Gene Symbol: OPRL1
• Gene ID (NCBI): 4987
• RRID: AB_10638603
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The OPRL1 (opioid related nociceptin receptor 1) is the G-protein coupled receptor activated by the endogenou NC (neuropeptide nociceptin) (PMID:7566152). OPRL1 is widely expressed in the central nervous system, including the hippocampal dentate gyrus, cortical areas, striatum, thalamus, and hypothalamus (PMID:19501074). OPRL1 has been found to play an essential role in cognition, and to modulate inflammation and immune responses (PMID:30152845).

Publications

Species	Application	Title
rat	WB,IHC	Int Immunopharmacol; PMID: 31865244; Authors - Neili Xu
human	WB	Cell Death Discov; The nociceptin receptor promotes autophagy through NF-kB signaling and is transcriptionally regulated by E2F1 in HCC.; Authors - Xiaoshuang Zhou ; KD Validated
rat	WB	Biomed Res Int; PMID: 25276817; Authors - Katarzyna Popiolek-Barczyk
rat		Biochim Biophys Acta; Pharmacological blockade of CXCR3 by (±)-NBI-74330 reduces neuropathic pain and enhances opioid effectiveness - Evidence from in vivo and in vitro studies.; Authors - Anna Piotrowska