OPTN/Optineurin Polyclonal antibody, PBS Only

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• OPTN/Optineurin Polyclonal antibody, PBS Only (10837-1-PBS)

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  WB analysis using 10837-1-AP (same clone as 10837-1-PBS)

  various lysates were subjected to SDS PAGE followed by western blot with 10837-1-AP (OPTN antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10837-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 10837-1-AP (same clone as 10837-1-PBS)

  WB result of OPTN antibody (10837-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OPTN transfected HeLa cells. This data was developed using the same antibody clone with 10837-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 10837-1-AP (same clone as 10837-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10837-1-AP (OPTN/Optineurin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10837-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 10837-1-AP (same clone as 10837-1-PBS)

  IP result of anti-OPTN/Optineurin (IP:10837-1-AP, 4ug; Detection:10837-1-AP 1:10000) with HeLa cells lysate 1400 ug. This data was developed using the same antibody clone with 10837-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 10837-1-AP (same clone as 10837-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10837-1-AP (OPTN Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 10837-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 10837-1-AP (same clone as 10837-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10837-1-AP (OPTN Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 10837-1-PBS in a different storage buffer formulation.

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  IF Staining of rat eye using 10837-1-AP (same clone as 10837-1-PBS)

  Immunofluorescent analysis of (-20°C Acetone) fixed paraffin-embedded rat eye tissue using OPTN antibody (10837-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10837-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 10837-1-AP (same clone as 10837-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using OPTN antibody (10837-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 10837-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 10837-1-AP (same clone as 10837-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human OPTN (10837-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 10837-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

OPTN/Optineurin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA

Cat No. 10837-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA

Synonyms

Optineurin, OPTN, ALS12, E3-14.7K-interacting protein, FAA4

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Product Information

10837-1-PBS targets OPTN/Optineurin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1272

  Product name: Recombinant human OPTN protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 233-577 aa of BC013876

  Sequence: VSQLLLCLREGNQKVERLEVALKEAKERVSDFEKKTSNRSEIETQTEGSTEKENDEEKGPETVGSEVEALNLQVTSLFKELQEAHTKLSEAELMKKRLQEKCQALERKNSAIPSELNEKQELVYTNKKLELQVESMLSEIKMEQAKTEDEKSKLTVLQMTHNKLLQEHNNALKTIEELTRKESEKVDRAVLKELSEKLELAEKALASKQLQMDEMKQTIAKQEEDLETMTILRAQMEVYCSDFHAERAAREKIHEEKEQLALQLAVLLKENDAFEDGGRQSLMEMQSRHGARTSDSDQQAYLVQRGAEDRDWRQQRNIPIHSCPKCGEVLPDIDTLQIHVMDCII

• Full Name: optineurin
• Calculated Molecular Weight: 66 kDa
• Observed Molecular Weight: 66-70 kDa
• GenBank Accession Number: BC013876
• Gene Symbol: OPTN
• Gene ID (NCBI): 10133
• RRID: AB_2156665
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96CV9
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

OPTN, also named as FIP2, GLC1E, HIP7, HYPL and NRP, plays a neuroprotective role in the eye and optic nerve. It is probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. OPTN may act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). OPTN may constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death. Defects in OPTN are a cause of susceptibility to normal pressure glaucoma (NPG). OPTN mutated in adult-onset primary open angle glaucoma. It supports the protective role of OPTN in the trabecular meshwork. OPTN has 3 isoforms with MW 66,65 and 60 kDa (refer to UniProt). Catalog#10837-1-AP recognises 66 and 70-74 kDa band, and the additional 70-74 kDa band due to phosphorylation.