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OSBPL10 Polyclonal antibody, PBS Only (15491-1-PBS)

OSBPL10 Polyclonal antibody, PBS Only

Name: OSBPL10 Polyclonal antibody, PBS Only,
Brand: Proteintech

OSBPL10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 15491-1-PBS

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

ORP 10, ORP10, ORP-10, OSBP related protein 10, OSBP9

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

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-/ -

Freight/Packing: -

Quantity

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Filter: AllWBIPIHCIF/ICC

WB analysis of HepG2 using 15491-1-AP (same clone as 15491-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 15491-1-AP (OSBPL10 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15491-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of HeLa cells using 15491-1-AP (same clone as 15491-1-PBS)
IP result of anti-OSBPL10 (15491-1-AP for IP and Detection). This data was developed using the same antibody clone with 15491-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 15491-1-AP (same clone as 15491-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer using 15491-1-AP (OSBPL10 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15491-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 15491-1-AP (same clone as 15491-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer using 15491-1-AP (OSBPL10 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15491-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 15491-1-AP (same clone as 15491-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15491-1-AP (OSBPL10 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15491-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 15491-1-AP (same clone as 15491-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15491-1-AP (OSBPL10 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15491-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human rectal cancer using 15491-1-AP (same clone as 15491-1-PBS)
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 15491-1-AP (OSBPL10 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15491-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human rectal cancer using 15491-1-AP (same clone as 15491-1-PBS)
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 15491-1-AP (OSBPL10 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15491-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using 15491-1-AP (same clone as 15491-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using OSBPL10 antibody (15491-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 15491-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information

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Product Information

15491-1-PBS targets OSBPL10 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7861 Product name: Recombinant human OSBPL10 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-348 aa of BC003168 Sequence: MERAVQGTDGGGGSNSSSRSSSRATSAGSSPSCSLAGRGVSSRSAAAGLGGGGSRSSPGSVAASPSGGGGRRREPALEGVLSKYTNLLQGWQNRYFVLDFEAGILQYFVNEQSKHQKPRGVLSLSGAIVSLSDEAPHMLVVYSANGEMFKLRAADAKEKQFWVTQLRACAKYHMEMNSKSAPSSRSRSLTLLPHGTPNSASPCSQRHLSVGAPGVVTITHHKSPAAARRAKSQYSGQLHEVREMMNQVEGQQKNLVHAIESLPGSGPLTALDQDLLLLKATSAATLSCLGECLNLLQQSVHQAGQPSQKPGASENILGWHGSKSHSTEQLKNGTLGSLPSASANITWA Predict reactive species
Full Name	oxysterol binding protein-like 10
Calculated Molecular Weight	84 kDa
Observed Molecular Weight	75-80 kDa
GenBank Accession Number	BC003168
Gene Symbol	OSBPL10
Gene ID (NCBI)	114884
RRID	AB_2158356
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BXB5
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

OSBPL10, also known as ORP10, is a member of the oxysterol-binding protein family. OSBPL10 localizes on microtubules and acts as a regulator of cellular lipid biosyntheses and apolipoprotein B-100. Genetic variation in OSBPL10 is associated with dyslipidmias and peripheral artery disease.