OSBPL2 Polyclonal antibody

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• OSBPL2 Polyclonal antibody (14751-1-AP)

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• KD/KO Validated

OSBPL2 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Cat No. 14751-1-AP Copied!

Publications (16)

Reviews (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, IP, ChIP, ELISA

Synonyms

OSBP-related protein 2, OSBP related protein 2, ORP-2, ORP2, ORP 2

• 

  WB analysis using 14751-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14751-1-AP (OSBPL2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A549 using 14751-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14751-1-AP (OSBPL2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 14751-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14751-1-AP (OSBPL2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 14751-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14751-1-AP (OSBPL2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HuH-7 using 14751-1-AP

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 14751-1-AP (OSBPL2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 14751-1-AP

  IP result of anti-OSBPL2 (IP:14751-1-AP, 4ug; Detection:14751-1-AP 1:500) with HeLa cells lysate 880ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary cancer using 14751-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 14751-1-AP (OSBPL2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of mouse brain using 14751-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using OSBPL2 antibody (14751-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, HuH-7 cells, mouse brain tissue, HepG2 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14751-1-AP targets OSBPL2 in WB, IHC, IF-P, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6496

  Product name: Recombinant human OSBPL2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 128-480 aa of BC000296

  Sequence: ERMQSVAAFAVSAVASQWERTGKPFNPLLGETYELIREDLGFRFISEQVSHHPPISAFHSEGLNHDFLFHGSIYPKLKFWGKSVEAEPRGTITLELLKHNEAYTWTNPTCCVHNVIIGKLWIEQYGTVEILNHRTGHKCVLHFKPCGLFGKELHKVEGHIQDKNKKKLFMIYGKWTECLWGIDPVSYESFKKQERRGDHLRKAKLDEDSGKADSDVADDVPVAQETVQVIPGSKLLWRINTRPPNSAQMYNFTSFTVSLNELETGMEKTLPPTDCRLRPDIRGMENGNMDLASQEKERLEEKQREARRERAKEEAEWQTRWFYPGNNPYTGTPDWLYAGDYFERNFSDCPDIY

• Full Name: oxysterol binding protein-like 2
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 51 kDa, 56 kDa
• GenBank Accession Number: BC000296
• Gene Symbol: OSBPL2
• Gene ID (NCBI): 9885
• RRID: AB_2156092
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H1P3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The anti-OSBPL2 antibody (Cat# 14751-1-AP) has 16 total citations published in journals including Autophagy, Protein & Cell, Molecular Cell, Cell Reports, JCI Insight, Genetics in Medicine, Hepatology Communications, Cell Signaling, iScience, Journal of Biological Chemistry, Journal of Biomedical Research, Experimental Cell Research, Molecular and Cellular Endocrinology, Hearing Research, PLoS ONE, and PNAS. Research fields span autophagy, lipophagy, cholesterol homeostasis, hearing loss, liver disease (MASH/MASLD), ferroptosis, lipid droplet biology, and cytoskeletal regulation.

Automatically generated from the 16 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IF	Autophagy; OSBPL2 mutations impair autophagy and lead to hearing loss, potentially remedied by rapamycin.; Authors - Young Ik Koh
human	WB	Protein Cell; ORP8 acts as a lipophagy receptor to mediate lipid droplet turnover; Authors - Maomao Pu
human	wb	Mol Cell; ORP2 Delivers Cholesterol to the Plasma Membrane in Exchange for Phosphatidylinositol 4, 5-Bisphosphate (PI(4,5)P2).; Authors - Huan Wang ; KD Validated
mouse	WB	Cell Rep; Niemann-Pick Type C Disease Reveals a Link between Lysosomal Cholesterol and PtdIns(4,5)P2 That Regulates Neuronal Excitability.; Authors - Oscar Vivas
mouse	IF	JCI Insight; Mutations in OSBPL2 cause hearing loss associated with primary cilia defects via Sonic Hedgehog signaling.; Authors - Hairong Shi
	WB	Genet Med; Identification of OSBPL2 as a novel candidate gene for progressive nonsyndromic hearing loss by whole-exome sequencing.; Authors - Guangqian Xing

Reviews

5.0 out of 5

1 verified customer reviews

What customers say

The single review for 14751-1-AP is from Boyan Zhang (November 2024), who rated the product 5 stars. The reviewer used it for Western Blot and reported that it recognized a band at the expected molecular weight, indicating reliable performance for their application.

Automatically generated from the 1 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Boyan (Verified Customer) (11-25-2024)

recognized a band at the expected molecular weight

• Applications: Western Blot