OSBPL2 Polyclonal antibody

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  WB analysis using 14751-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14751-1-AP (OSBPL2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 14751-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14751-1-AP (OSBPL2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14751-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14751-1-AP (OSBPL2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HuH-7 using 14751-1-AP

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 14751-1-AP (OSBPL2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14751-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14751-1-AP (OSBPL2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14751-1-AP

  IP result of anti-OSBPL2 (IP:14751-1-AP, 4ug; Detection:14751-1-AP 1:500) with HeLa cells lysate 880ug.

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  IHC staining of human ovary cancer using 14751-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 14751-1-AP (OSBPL2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 14751-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using OSBPL2 antibody (14751-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

OSBPL2 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Cat No. 14751-1-AP

Publications(11)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, IP, ChIP, ELISA

Synonyms

OSBP-related protein 2, OSBP related protein 2, ORP-2, ORP2, ORP 2

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, HuH-7 cells, mouse brain tissue, HepG2 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IF: See 5 publications below
• ChIP: See 1 publications below

Product Information

14751-1-AP targets OSBPL2 in WB, IHC, IF-P, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6496

  Product name: Recombinant human OSBPL2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 128-480 aa of BC000296

  Sequence: ERMQSVAAFAVSAVASQWERTGKPFNPLLGETYELIREDLGFRFISEQVSHHPPISAFHSEGLNHDFLFHGSIYPKLKFWGKSVEAEPRGTITLELLKHNEAYTWTNPTCCVHNVIIGKLWIEQYGTVEILNHRTGHKCVLHFKPCGLFGKELHKVEGHIQDKNKKKLFMIYGKWTECLWGIDPVSYESFKKQERRGDHLRKAKLDEDSGKADSDVADDVPVAQETVQVIPGSKLLWRINTRPPNSAQMYNFTSFTVSLNELETGMEKTLPPTDCRLRPDIRGMENGNMDLASQEKERLEEKQREARRERAKEEAEWQTRWFYPGNNPYTGTPDWLYAGDYFERNFSDCPDIY

• Full Name: oxysterol binding protein-like 2
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 51 kDa, 56 kDa
• GenBank Accession Number: BC000296
• Gene Symbol: OSBPL2
• Gene ID (NCBI): 9885
• RRID: AB_2156092
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H1P3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Protein Cell; ORP8 acts as a lipophagy receptor to mediate lipid droplet turnover; Authors - Maomao Pu
human	WB,IF	Autophagy; OSBPL2 mutations impair autophagy and lead to hearing loss, potentially remedied by rapamycin.; Authors - Young Ik Koh
mouse	IF	JCI Insight; Mutations in OSBPL2 cause hearing loss associated with primary cilia defects via Sonic Hedgehog signaling.; Authors - Hairong Shi
	WB	Genet Med; Identification of OSBPL2 as a novel candidate gene for progressive nonsyndromic hearing loss by whole-exome sequencing.; Authors - Guangqian Xing
human	IF,ChIP	Mol Cell Endocrinol; Oxysterol-related-binding-protein related Protein-2 (ORP2) regulates cortisol biosynthesis and cholesterol homeostasis.; Authors - Tamara Escajadillo ; KD Validated
human	WB	Exp Cell Res; OSBPL2 deficiency upregulate SQLE expression increasing intracellular cholesterol and cholesteryl ester by AMPK/SP1 and SREBF2 signalling pathway.; Authors - Cui Zhang ; KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (11-25-2024)

recognized a band at the expected molecular weight

• Applications: Western Blot