OSGEP Polyclonal antibody

OSGEP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 15033-1-AP

Synonyms

GCPL1, hOSGEP, KAE1, OSGEP, OSGEP1, PRSMG1

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Validation Data Gallery

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  WB analysis using 15033-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15033-1-AP (OSGEP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 15033-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 15033-1-AP (OSGEP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 15033-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15033-1-AP (OSGEP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 15033-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15033-1-AP (OSGEP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 15033-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15033-1-AP (OSGEP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 15033-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15033-1-AP (OSGEP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 15033-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15033-1-AP (OSGEP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 15033-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15033-1-AP (OSGEP Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 15033-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15033-1-AP (OSGEP Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: PC-3 cells, human heart tissue, Raji cells, mouse colon tissue
• Positive IHC detected in: human kidney tissue, human heart tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

15033-1-AP targets OSGEP in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OSGEP fusion protein Ag4308
• Full Name: O-sialoglycoprotein endopeptidase
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC032310
• Gene Symbol: OSGEP
• Gene ID (NCBI): 55644
• RRID: AB_2283171
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OSGEP(O-sialoglycoprotein endopeptidase) is also named as hOSGEP, GCPL1 and belongs to the KAE1 / YgjD family. The deduced 335-amino acid OSGEP protein has a predicted molecular mass of 36.4 kDa. It is a protease whose target is O-linked sialic acid-containing glycoproteins, such as episialin(PMID:10859271). It is involved in the metabolism of glycated proteins as a glycoprotein-related chaperon protease(PMID: 20824107).

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; Commonality and diversity in tRNA substrate recognition in t6A biogenesis by eukaryotic KEOPSs.; Authors - Jin-Tao Wang