OSGEPL1 Polyclonal antibody

OSGEPL1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 25694-1-AP

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

OSGEPL1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHC

WB analysis of HeLa using 25694-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 25694-1-AP (OSGEPL1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of NIH/3T3 using 25694-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25694-1-AP (OSGEPL1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of MCF-7 using 25694-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 25694-1-AP (OSGEPL1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 25694-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25694-1-AP (OSGEPL1 Antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 25694-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25694-1-AP (OSGEPL1 Antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human prostate hyperplasia using 25694-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 25694-1-AP (OSGEPL1 Antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human prostate hyperplasia using 25694-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 25694-1-AP (OSGEPL1 Antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, MCF-7 cells, NIH/3T3 cells
Positive IHC detected in	human colon tissue, human prostate hyperplasia tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25694-1-AP targets OSGEPL1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag22204 Product name: Recombinant human OSGEPL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-261 aa of BC011904 Sequence: MLILTKTAGVFFKPSKRKVYEFLRSFNFHPGTLFLHKIVLGIETSCDDTAAAVVDETGNVLGEAIHSQTEVHLKTGGIVPPAAQQLHRENIQRIVQEALSASGVSPSDLSAIATTIKPGLALSLGVGLSFSLQLVGQLKKPFIPIHHMEAHALTIRLTNKVEFPFLVLLISGGHCLLALVQGVSDFLLLGKSLDIAPGDMLDKVARRLSLIKHPECSTMSGGKAIEHLAKQGNRFHFDIKPPLHHAKNCDFSFTGLQHVTD Predict reactive species
Full Name	O-sialoglycoprotein endopeptidase-like 1
Calculated Molecular Weight	414 aa, 45 kDa
Observed Molecular Weight	45 kDa
GenBank Accession Number	BC011904
Gene Symbol	OSGEPL1
Gene ID (NCBI)	64172
RRID	AB_2880198
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9H4B0
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for OSGEPL1 antibody 25694-1-AP	Download protocol
WB protocol for OSGEPL1 antibody 25694-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The OSGEPL1 Polyclonal antibody (Cat# 25694-1-AP) has 1 citation. It was cited in Nature Communications in a 2018 study on CO₂-sensitive tRNA modification associated with human mitochondrial disease, where it was used for Western blot in human samples. The research field encompasses mitochondrial biology and RNA modification.