OSGEPL1 Polyclonal antibody

OSGEPL1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 25694-1-AP

Synonyms

OSGEPL1

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Validation Data Gallery

• 

  WB analysis of HeLa using 25694-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25694-1-AP (OSGEPL1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 25694-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25694-1-AP (OSGEPL1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 25694-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25694-1-AP (OSGEPL1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 25694-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25694-1-AP (OSGEPL1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 25694-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25694-1-AP (OSGEPL1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate hyperplasia using 25694-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 25694-1-AP (OSGEPL1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate hyperplasia using 25694-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 25694-1-AP (OSGEPL1 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, NIH/3T3 cells
• Positive IHC detected in: human colon tissue, human prostate hyperplasia tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 25694-1-AP is OSGEPL1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OSGEPL1 fusion protein Ag22204
• Full Name: O-sialoglycoprotein endopeptidase-like 1
• Calculated Molecular Weight: 414 aa, 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC011904
• Gene Symbol: OSGEPL1
• Gene ID (NCBI): 64172
• RRID: AB_2880198
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nat Commun; CO2-sensitive tRNA modification associated with human mitochondrial disease.; Authors - Huan Lin