OSTM1 Polyclonal antibody

OSTM1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 14621-1-AP

Synonyms

UNQ6098/PRO21201, Osteopetrosis-associated transmembrane protein 1, HSPC019, GL, Chloride channel 7 beta subunit

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Validation Data Gallery

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  WB analysis using 14621-1-AP

  mouse brain membrane protein lysates were subjected to SDS PAGE followed by western blot with 14621-1-AP (OSTM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 14621-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14621-1-AP (OSTM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 14621-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14621-1-AP (OSTM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 14621-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using OSTM1 antibody (14621-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: 37°C incubated mouse brain tissue, mouse brain membrane protein
• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

14621-1-AP targets OSTM1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OSTM1 fusion protein Ag6254
• Full Name: osteopetrosis associated transmembrane protein 1
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: ~ 60 kDa
• GenBank Accession Number: BC068581
• Gene Symbol: OSTM1
• Gene ID (NCBI): 28962
• RRID: AB_2298955
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OSTM1 is a type I transmembrane protein. Ostm1 is essential for osteoclast maturation and is responsible for the most severe forms of osteoporosis when mutated in mice and humans. The OSTM1 protein has 10 N-glycosylation sites and has an apparent mass of ~60 kDa after being highly N-glycosylated (PMID: 26598607; PMID: 32764302). The OSTM1 protein can also be detected at ~30-45 kDa and ~70-80 kDa in some literatures (PMID: 16525474; PMID: 24159188;PMID: 20830208).

Publications

Species	Application	Title
human	WB	J Virol; Osteopetrosis-Associated Transmembrane Protein 1 Recruits RNA Exosome To Restrict Hepatitis B Virus Replication.; Authors - Chunqiang Ma
mouse	WB	Biology (Basel); Leucine Repeat Rich Kinase 1 Controls Osteoclast Activity by Managing Lysosomal Trafficking and Secretion; Authors - Sandi Shen