OTC Polyclonal antibody

OTC Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 26470-1-AP

Synonyms

OCTD, ornithine carbamoyltransferase, Ornithine transcarbamylase, OTC, OTCase



Tested Applications

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

26470-1-AP targets OTC in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OTC fusion protein Ag24186
Full Name ornithine carbamoyltransferase
Calculated molecular weight 354 aa, 40 kDa
Observed molecular weight 36-40 kDa
GenBank accession numberBC074745
Gene symbol OTC
Gene ID (NCBI) 5009
RRIDAB_2880528
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ornithine transcarbamylase (OTC) is a liver and intestinal mucosa-located, intramitochondrial, rate-limiting enzyme in the urea cycle. It catalyzes the reaction that converts ornithine and carbamoyl phosphate into citrulline, which is the second step of the urea cycle, to detoxify the ammonia produced from amino acid catabolism.

Protocols

Product Specific Protocols
WB protocol for OTC antibody 26470-1-APDownload protocol
IHC protocol for OTC antibody 26470-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Autophagy

PHB2 (prohibitin 2) promotes PINK1-PRKN/Parkin-dependent mitophagy by the PARL-PGAM5-PINK1 axis.

Authors - Chaojun Yan
mouseWB

Mol Metab

SWI/SNF complex subunit BAF60a represses hepatic ureagenesis through a crosstalk between YB-1 and PGC-1α.

Authors - Wenxiang Zhang
humanWB

Front Cell Dev Biol

Urea as a By-Product of Ammonia Metabolism Can Be a Potential Serum Biomarker of Hepatocellular Carcinoma.

Authors - Changsen Bai
humanWB

Biochem Biophys Res Commun

Circadian oscillation expression of ornithine carbamoyltransferase and its significance in sleep disturbance.

Authors - Wei Wang
mouseWB,IF

J Clin Invest

Glutamine synthetase limits b-catenin-mutated liver cancer growth by maintaining nitrogen homeostasis and suppressing mTORC1

Authors - Weiwei Dai
humanWB

Metab Brain Dis

p53 inhibits the Urea cycle and represses polyamine biosynthesis in glioma cell lines

Authors - Yuhong Zhao