OTC Polyclonal antibody

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• OTC Polyclonal antibody (26470-1-AP)

OTC Polyclonal Antibody for WB, IHC, ELISA

Cat No. 26470-1-AP Copied!

Publications (14)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

OCTD, ornithine carbamoyltransferase, Ornithine transcarbamylase, OTC, OTCase

• 

  WB analysis using 26470-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26470-1-AP (OTC antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 26470-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26470-1-AP (OTC antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 26470-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26470-1-AP (OTC antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 26470-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26470-1-AP (OTC antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse liver tissue, rat liver tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26470-1-AP targets OTC in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag24186

  Product name: Recombinant human OTC protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 35-354 aa of BC074745

  Sequence: VQLKGRDLLTLKNFTGEEIKYMLWLSADLKFRIKQKGEYLPLLQGKSLGMIFEKRSTRTRLSTETGFALLGGHPCFLTTQDIHLGVNESLTDTARVLSSMADAVLARVYKQSDLDTLAKEASIPIINGLSDLYHPIQILADYLTLQEHYSSLKGLTLSWIGDGNNILHSIMMSAAKFGMHLQAATPKGYEPDASVTKLAEQYAKENGTKLLLTNDPLEAAHGGNVLITDTWISMGQEEEKKKRLQAFQGYQVTMKTAKVAASDWTFLHCLPRKPEEVDDEVFYSPRSLVFPEAENRKWTIMAVMVSLLTDYSPQLQKPKF

• Full Name: ornithine carbamoyltransferase
• Calculated Molecular Weight: 354 aa, 40 kDa
• Observed Molecular Weight: 36-40 kDa
• GenBank Accession Number: BC074745
• Gene Symbol: OTC
• Gene ID (NCBI): 5009
• RRID: AB_2880528
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P00480
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ornithine transcarbamylase (OTC) is a liver and intestinal mucosa-located, intramitochondrial, rate-limiting enzyme in the urea cycle. It catalyzes the reaction that converts ornithine and carbamoyl phosphate into citrulline, which is the second step of the urea cycle, to detoxify the ammonia produced from amino acid catabolism.

Publications

What published studies show

OTC antibody (Cat# 26470-1-AP) has 14 citations across journals including Nature, Cancer Research, Autophagy, J Clin Invest, Adv Sci, Sci Adv, Cancer Lett, Dev Cell, EMBO Mol Med, Mol Metab, Front Cell Dev Biol, Brain Res Bull, Metab Brain Dis, and BBRC. Research fields span ferroptosis, colorectal/liver/lung/glioma cancers, hepatocellular carcinoma, mitophagy, mitochondrial myopathy, urea cycle and nitrogen metabolism, circadian rhythm, and epilepsy.

Automatically generated from the 14 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Nature; Spermine is an endogenous iron chelator that inhibits ferroptosis; Authors - Man Li
human	IHC	Cancer Res; Arginine Deprivation Induces Quiescence and Confers Vulnerability to Ferroptosis in Colorectal Cancer; Authors - Yanyun Lin
human	WB	Autophagy; PHB2 (prohibitin 2) promotes PINK1-PRKN/Parkin-dependent mitophagy by the PARL-PGAM5-PINK1 axis.; Authors - Chaojun Yan
mouse	WB,IF	J Clin Invest; Glutamine synthetase limits b-catenin-mutated liver cancer growth by maintaining nitrogen homeostasis and suppressing mTORC1; Authors - Weiwei Dai
		Adv Sci (Weinh); PFKFB4 Deubiquitination by USP10 Enhances Fumarate Metabolism to Orchestrate the KDM1A/Rad51 Axis and Confer Radioresistance in Lung Cancer.; Authors - Yunshang Chen
human, mouse	WB,IHC,IF	Sci Adv; Impaired nitrogenous waste clearance promotes hepatocellular carcinoma.; Authors - Xinlu Han