OTC Polyclonal antibody

OTC Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 26470-1-AP

Synonyms

OCTD, ornithine carbamoyltransferase, Ornithine transcarbamylase, OTC, OTCase

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Validation Data Gallery

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  WB analysis using 26470-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26470-1-AP (OTC antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26470-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26470-1-AP (OTC antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 26470-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26470-1-AP (OTC antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 26470-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26470-1-AP (OTC antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse liver tissue, rat liver tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IF: See 1 publications below

Product Information

26470-1-AP targets OTC in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OTC fusion protein Ag24186
• Full Name: ornithine carbamoyltransferase
• Calculated Molecular Weight: 354 aa, 40 kDa
• Observed Molecular Weight: 36-40 kDa
• GenBank Accession Number: BC074745
• Gene Symbol: OTC
• Gene ID (NCBI): 5009
• RRID: AB_2880528
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ornithine transcarbamylase (OTC) is a liver and intestinal mucosa-located, intramitochondrial, rate-limiting enzyme in the urea cycle. It catalyzes the reaction that converts ornithine and carbamoyl phosphate into citrulline, which is the second step of the urea cycle, to detoxify the ammonia produced from amino acid catabolism.

Publications

Species	Application	Title
human	WB	Autophagy; PHB2 (prohibitin 2) promotes PINK1-PRKN/Parkin-dependent mitophagy by the PARL-PGAM5-PINK1 axis.; Authors - Chaojun Yan
mouse	WB	Mol Metab; SWI/SNF complex subunit BAF60a represses hepatic ureagenesis through a crosstalk between YB-1 and PGC-1α.; Authors - Wenxiang Zhang
human	WB	Front Cell Dev Biol; Urea as a By-Product of Ammonia Metabolism Can Be a Potential Serum Biomarker of Hepatocellular Carcinoma.; Authors - Changsen Bai
human	WB	Biochem Biophys Res Commun; Circadian oscillation expression of ornithine carbamoyltransferase and its significance in sleep disturbance.; Authors - Wei Wang
mouse	WB,IF	J Clin Invest; Glutamine synthetase limits b-catenin-mutated liver cancer growth by maintaining nitrogen homeostasis and suppressing mTORC1; Authors - Weiwei Dai
human	WB	Metab Brain Dis; p53 inhibits the Urea cycle and represses polyamine biosynthesis in glioma cell lines; Authors - Yuhong Zhao