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OTUD1 Polyclonal antibody, PBS Only (29921-1-PBS)

Filter: AllWBIHC

WB analysis using 29921-1-AP (same clone as 29921-1-PBS)
A549 cells were subjected to SDS PAGE followed by western blot with 29921-1-AP (OTUD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29921-1-PBS in a different storage buffer formulation.
WB analysis of mouse colon using 29921-1-AP (same clone as 29921-1-PBS)
mouse colon tissue were subjected to SDS PAGE followed by western blot with 29921-1-AP (OTUD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29921-1-PBS in a different storage buffer formulation.
WB analysis using 29921-1-AP (same clone as 29921-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 29921-1-AP (OTUD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29921-1-PBS in a different storage buffer formulation.
IHC staining of human cervical cancer using 29921-1-AP (same clone as 29921-1-PBS)
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 29921-1-AP (OTUD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29921-1-PBS in a different storage buffer formulation.
IHC staining of human ovary tumor using 29921-1-AP (same clone as 29921-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 29921-1-AP (OTUD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29921-1-PBS in a different storage buffer formulation.
IHC staining of mouse liver using 29921-1-AP (same clone as 29921-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29921-1-AP (OTUD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29921-1-PBS in a different storage buffer formulation.
IHC staining of human ovary tumor using 29921-1-AP (same clone as 29921-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 29921-1-AP (OTUD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29921-1-PBS in a different storage buffer formulation.
IHC staining of human cervical cancer using 29921-1-AP (same clone as 29921-1-PBS)
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 29921-1-AP (OTUD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29921-1-PBS in a different storage buffer formulation.

OTUD1 Polyclonal antibody, PBS Only

Name: OTUD1 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 29921-1-PBS

OTUD1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 29921-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

29921-1-PBS targets OTUD1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag31996 Product name: Recombinant human OTUD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 272-431 aa of Sequence: SSAAEPVIVSRSDPRDEKLALYLAEVEKQDKYLRQRNKYRFHIIPDGNCLYRAVSKTVYGDQSLHRELREQTVHYIADHLDHFSPLIEGDVGEFIIAAAQDGAWAGYPELLAMGQMLNVNIHLTTGGRLESPTVSTMIHYLGPEDSLRPSIWLSWLSNGH Predict reactive species
Full Name	OTU domain containing 1
Calculated Molecular Weight	51 kDa
Observed Molecular Weight	50-55 kDa
Gene Symbol	OTUD1
Gene ID (NCBI)	220213
RRID	AB_3086190
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q5VV17
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

OTUD1, OTU domain-containing protein 1, is a deubiquitinating enzyme that specifically hydrolyzes 'Lys-63'-linked polyubiquitin to monoubiquitin (PMID: 23827681). OTUD1-mediated deubiquitination prevents activation of the ribosome quality control and subsequent dissociation of ribosomes and stimulates formation of polysomes and translation (PMID: 36445135). OTUD1 is a colon-specific deubiquitinase that expressed in normal colonic epithelium goblet cells (PMID: 36910614).