OTUD3 Polyclonal antibody, PBS Only

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• OTUD3 Polyclonal antibody, PBS Only (29622-1-PBS)

• 

  WB analysis of HeLa using 29622-1-AP (same clone as 29622-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29622-1-AP (OTUD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29622-1-PBS in a different storage buffer formulation.

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  WB analysis of HCT 116 using 29622-1-AP (same clone as 29622-1-PBS)

  WB result of OTUD3 antibody (29622-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OTUD3 transfected HCT 116 cells. This data was developed using the same antibody clone with 29622-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 29622-1-AP (same clone as 29622-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29622-1-AP (OTUD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29622-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

OTUD3 Polyclonal Antibody for WB, Indirect ELISA

Cat No. 29622-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

DUBA4, KIAA0459, OTU domain containing 3, RP11 460G22.1

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Product Information

29622-1-PBS targets OTUD3 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag30425

  Product name: Recombinant human OTUD3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 192-398 aa of NM_015207

  Sequence: NSEAPAHLQTDFQMLHQDESNKREKIKTKGMDSEDDLRDEVEDAVQKVCNATGCSDFNLIVQNLEAENYNIESAIIAVLRMNQGKRNNAEENLEPSGRVLKQCGPLWEEGGSGARIFGNQGLNEGRTENNKAQASPSEENKANKNQLAKVTNKQRREQQWMEKKKRQEERHRHKALESRGSHRDNNRSEAEANTQVTLVKTFAALNI

• Full Name: OTU domain containing 3
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: NM_015207
• Gene Symbol: OTUD3
• Gene ID (NCBI): 23252
• RRID: AB_2918332
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5T2D3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

OTUD3 is a kind of deubiquitinating enzyme that hydrolyzes 'Lys-6'- and 'Lys-11'-linked polyubiquitin. It's an important regulator of energy metabolism. Glucose and fatty acids trigger its nuclear translocation by CBP-dependent acetylation. In the nucleus, deubiquitinates and stabilizes the nuclear receptor PPARD regulating the expression of various genes involved in glucose and lipid metabolism and oxidative phosphorylation. The calculated MW of OTUD3 is 45 kDa, 29622-1-AP can detect the band around 43 kDa. (PubMed:23827681, 35675826).