OTUD3 Recombinant monoclonal antibody

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  WB analysis of HeLa using 84717-1-RR

  HeLa cells were subjected to SDS PAGE followed by western blot with 84717-1-RR (OTUD3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 84717-1-RR

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 84717-1-RR (OTUD3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HCT 116 using 84717-1-RR

  WB result of OTUD3 antibody (84717-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OTUD3 transfected HCT 116 cells.

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  IHC staining of mouse cerebellum using 84717-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 84717-1-RR (OTUD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84717-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84717-1-RR against Human OTUD3 were performed. The affinity constant is below 1 pM.

• Featured Product
• KD/KO Validated

OTUD3 Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 84717-1-RR

Clone No.242202B11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

242202B11, DUBA4, KIAA0459, OTU domain containing 3, RP11 460G22.1

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Tested Applications

• Positive WB detected in: HeLa cells, HCT 116 cells, Neuro-2a cells, HepG2 cells, MCF-7 cells
• Positive IHC detected in: mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84717-1-RR targets OTUD3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag30425

  Product name: Recombinant human OTUD3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 192-398 aa of NM_015207

  Sequence: NSEAPAHLQTDFQMLHQDESNKREKIKTKGMDSEDDLRDEVEDAVQKVCNATGCSDFNLIVQNLEAENYNIESAIIAVLRMNQGKRNNAEENLEPSGRVLKQCGPLWEEGGSGARIFGNQGLNEGRTENNKAQASPSEENKANKNQLAKVTNKQRREQQWMEKKKRQEERHRHKALESRGSHRDNNRSEAEANTQVTLVKTFAALNI

• Full Name: OTU domain containing 3
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: NM_015207
• Gene Symbol: OTUD3
• Gene ID (NCBI): 23252
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q5T2D3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OTUD3 is a kind of deubiquitinating enzyme that hydrolyzes 'Lys-6'- and 'Lys-11'-linked polyubiquitin. It's an important regulator of energy metabolism. Glucose and fatty acids trigger its nuclear translocation by CBP-dependent acetylation. In the nucleus, deubiquitinates and stabilizes the nuclear receptor PPARD regulating the expression of various genes involved in glucose and lipid metabolism and oxidative phosphorylation.