OTUD5 Recombinant monoclonal antibody

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• OTUD5 Recombinant monoclonal antibody (86490-1-RR)

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  WB analysis using 86490-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86490-1-RR (OTUD5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

OTUD5 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86490-1-RR

Clone No.251221B9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

DUBA, EC:3.4.19.12, OTU domain-containing protein 5, OTUD 5

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Tested Applications

• Positive WB detected in: Jurkat cells, K-562 cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86490-1-RR targets OTUD5 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag15190

  Product name: Recombinant human OTUD5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 202-561 aa of BC028225

  Sequence: WFEKALRDKKGFIIKQMKEDGACLFRAVADQVYGDQDMHEVVRKHCMDYLMKNADYFSNYVTEDFTTYINRKRKNNCHGNHIEMQAMAEMYNRPVEVYQYSTEPINTFHGIHQNEDEPIRVSYHRNIHYNSVVNPNKATIGVGLGLPSFKPGFAEQSLMKNAIKTSEESWIEQQMLEDKKRATDWEATNEAIEEQVARESYLQWLRDQEKQARQVRGPSQPRKASATCSSATAAASSGLEEWTSRSPRQRSSASSPEHPELHAELGMKPPSPGTVLALAKPPSPCAPGTSSQFSAGADRATSPLVSLYPALECRALIQQMSPSAFGLNDWDDDEILASVLAVSQQEYLDSMKKNKVPCRC

• Full Name: OTU domain containing 5
• Calculated Molecular Weight: 571 aa, 61 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC028225
• Gene Symbol: OTUD5
• Gene ID (NCBI): 55593
• RRID: AB_3744921
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96G74
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The deubiquitinating enzyme OTUD5, also known as deubiquitinating enzyme A (DUBA), is a member of the deubiquitinating enzyme OTU family. By deubiquitinating other proteins, OTUD5 is an important regulator of several physiological processes, such as immune signaling and DNA damage responses. The dysregulation of these processes can increase the risk of associated diseases, including inflammation, cancer and genetic disorders.