OTUD7B Monoclonal antibody

• Featured Product
• KD/KO Validated

OTUD7B Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

6

CloneNo.

2C12D9

Cat no : 66276-1-Ig

Synonyms

CEZANNE, OTU domain containing 7B, OTUD7B, ZA20D1, Zinc finger protein Cezanne

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Validation Data Gallery

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  WB analysis of Jurkat using 66276-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66276-1-Ig (OTUD7B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

66276-1-Ig targets OTUD7B in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: OTUD7B fusion protein Ag10513
• Full Name: OTU domain containing 7B
• Calculated Molecular Weight: 427aa,48 kDa; 843aa,93 kDa
• Observed Molecular Weight: 92 kDa
• GenBank Accession Number: BC020622
• Gene Symbol: OTUD7B
• Gene ID (NCBI): 56957
• RRID: AB_2881659
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OUTD7B is a member of the A20 family of the deubiquitinating cysteine proteases, which has deubiquitinating activity that is directed towards 'Lys-48' or 'Lys-63'-linked polyubiquitin chains. It plays a key role in the negative feedback regulation of NF-κB signaling in response to multiple stimuli and has been described as central gatekeeper in inflammation and immunity.

Publications

Species	Application	Title
human	WB	Nature; TRAF2 and OTUD7B govern a ubiquitin-dependent switch that regulates mTORC2 signalling.; Authors - Bin Wang ; KD Validated
human	WB,IF	Autophagy; High-throughput screening of functional deubiquitinating enzymes in autophagy.; Authors - Shuo Tian ; KO Validated
human	WB,IF	Autophagy; OTUD7B deubiquitinates SQSTM1/p62 and promotes IRF3 degradation to regulate antiviral immunity.; Authors - Weihong Xie ; KD Validated
human	WB	Mol Biol Rep; DJ-1 activates the noncanonical NF-κB pathway via interaction with Cezanne to inhibit the apoptosis and promote the proliferation of Ishikawa cells; Authors - Qi‑Zhou Zhu ; KD Validated
human	IHC	J Exp Clin Cancer Res; OTUD7B suppresses Smac mimetic-induced lung cancer cell invasion and migration via deubiquitinating TRAF3.; Authors - Boxiang Zhang
human	WB, IHC	Cell Death Dis; OTUD7B stabilizes estrogen receptor α and promotes breast cancer cell proliferation.; Authors - Jianing Tang