OTUD7B Monoclonal antibody, PBS Only (Capture)

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  WB analysis of Jurkat using 66276-1-Ig (same clone as 66276-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66276-1-Ig (OTUD7B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66276-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP51475-1

  Cytometric bead array sample test of MP51475-1, OTUD7B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66276-1-PBS. Detection antibody: 66276-2-PBS.

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  Cytometric bead array standard curve of MP51475-1

  Cytometric bead array standard curve of MP51475-1, OTUD7B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66276-1-PBS. Detection antibody: 66276-2-PBS. Standard:Ag10513. Range: 0.781-100 ng/mL.

• Featured Product
• KD/KO Validated

OTUD7B Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Cat No. 66276-1-PBS

Clone No.2C12D9

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

2C12D9, Cellular zinc finger anti-NF-kappa-B protein, Cezanne, EC:3.4.19.12, OTU domain containing 7B

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Product Information

66276-1-PBS targets OTUD7B as part of a matched antibody pair:

MP51475-1: 66276-1-PBS capture and 66276-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10513

  Product name: Recombinant human OTUD7B protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-355 aa of BC020622

  Sequence: MTLDMDAVLSDFVRSTGAEPGLARDLLEGKNWDVNAALSDFEQLRQVHAGNLPPSFSEGSGGSRTPEKGFSDREPTRPPRPILQRQDDIVQEKRLSRGISHASSSIVSLARSHVSSNGGGGGSNEHPLEMPICAFQLPDLTVYNEDFRSFIERDLIEQSMLVALEQAGRLNWWVSVDPTSQRLLPLATTGDGNCLLHAASLGMWGFHDRDLMLRKALYALMEKGVEKEALKRRWRWQQTQQNEESGLVYTEDEWQKEWNELIKLASSEPRMHLGTNGANCGGVESSEEPVYESLEEFHVFVLAHVLRRPIVVVADTMLRDSGGEAFAPIPFGGIYLPLEVPASQCHRSPLVLAYD

• Full Name: OTU domain containing 7B
• Calculated Molecular Weight: 427aa,48 kDa; 843aa,93 kDa
• Observed Molecular Weight: 92 kDa
• GenBank Accession Number: BC020622
• Gene Symbol: OTUD7B
• Gene ID (NCBI): 56957
• RRID: AB_2881659
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q6GQQ9
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

OUTD7B is a member of the A20 family of the deubiquitinating cysteine proteases, which has deubiquitinating activity that is directed towards 'Lys-48' or 'Lys-63'-linked polyubiquitin chains. It plays a key role in the negative feedback regulation of NF-κB signaling in response to multiple stimuli and has been described as central gatekeeper in inflammation and immunity.