OTX1 Polyclonal antibody

• Featured Product
• KD/KO Validated

OTX1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 26595-1-AP

Synonyms

Homeobox protein OTX1, orthodenticle homeobox 1, Orthodenticle homolog 1, OTX1

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Validation Data Gallery

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  WB analysis of HEK-293 using 26595-1-AP

  WB result of OTX1 antibody (26595-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-OTX1 transfected HEK-293 cells.

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  WB analysis of COLO 320 using 26595-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 26595-1-AP (OTX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 26595-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 26595-1-AP (OTX1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: COLO 320 cells, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26595-1-AP is OTX1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OTX1 fusion protein Ag24252
• Full Name: orthodenticle homeobox 1
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 37-43 kDa
• GenBank Accession Number: BC007621
• Gene Symbol: OTX1
• Gene ID (NCBI): 5013
• RRID: AB_2880568
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OTX1, also named as Orthodenticle homolog 1, is a 354 amino acid protein, which belongs to the paired homeobox family. OTX1 is expressed in brain and detected in the anterior part of the neural fetal retina. OTX1 probably plays a role in the development of the brain and the sense organs.