OXCT1 Monoclonal antibody, PBS Only

• 

  WB analysis of rat heart using 67836-1-Ig (same clone as 67836-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 67836-1-Ig (SCOT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67836-1-Ig (same clone as 67836-1-PBS)

  WB result of OXCT1 antibody (67836-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OXCT1 transfected HeLa cells. This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67836-1-Ig (same clone as 67836-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67836-1-Ig (SCOT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 67836-1-Ig (same clone as 67836-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67836-1-Ig (SCOT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 67836-1-Ig (same clone as 67836-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67836-1-Ig (SCOT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 67836-1-Ig (same clone as 67836-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67836-1-Ig (SCOT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 67836-1-Ig (same clone as 67836-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67836-1-Ig (SCOT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rabbit brain using 67836-1-Ig (same clone as 67836-1-PBS)

  rabbit brain tissue were subjected to SDS PAGE followed by western blot with 67836-1-Ig (SCOT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 67836-1-Ig (same clone as 67836-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67836-1-Ig (OXCT1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 67836-1-Ig (same clone as 67836-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using OXCT1 antibody (67836-1-Ig, Clone: 1G1B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). CL594-Phalloidin (red). This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 67836-1-Ig (same clone as 67836-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OXCT1 antibody (67836-1-Ig, Clone: 1G1B9 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 67836-1-Ig (same clone as 67836-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OXCT1 antibody (67836-1-Ig, Clone: 1G1B9 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 67836-1-Ig (same clone as 67836-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OXCT1 antibody (67836-1-Ig, Clone: 1G1B9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 67836-1-Ig (same clone as 67836-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OXCT1 antibody (67836-1-Ig, Clone: 1G1B9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 67836-1-Ig (same clone as 67836-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human OXCT1 (67836-1-Ig, Clone:1G1B9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

OXCT1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 67836-1-PBS

Clone No.1G1B9

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

1G1B9, 3 oxoacid CoA transferase 1, 3-oxoacid CoA-transferase 1, EC:2.8.3.5, OXCT

---

Product Information

67836-1-PBS targets OXCT1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag24792

  Product name: Recombinant human SCOT protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 151-520 aa of BC009001

  Sequence: GVPAFYTPTGYGTLVQEGGSPIKYNKDGSVAIASKPREVREFNGQHFILEEAITGDFALVKAWKADRAGNVIFRKSARNFNLPMCKAAETTVVEVEEIVDIGAFAPEDIHIPQIYVHRLIKGEKYEKRIERLSIRKEGDGEAKSAKPGDDVRERIIKRAALEFEDGMYANLGIGIPLLASNFISPNITVHLQSENGVLGLGPYPRQHEADADLINAGKETVTILPGASFFSSDESFAMIRGGHVDLTMLGAMQVSKYGDLANWMIPGKMVKGMGGAMDLVSSAKTKVVVTMEHSAKGNAHKIMEKCTLPLTGKQCVNRIITEKAVFDVDKKKGLTLIELWEGLTVDDVQKSTGCDFAVSPKLMPMQQIAN

• Full Name: 3-oxoacid CoA transferase 1
• Calculated Molecular Weight: 520 aa, 56 kDa
• Observed Molecular Weight: 52-56 kDa
• GenBank Accession Number: BC009001
• Gene Symbol: OXCT1
• Gene ID (NCBI): 5019
• RRID: AB_2918598
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P55809
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

OXCT1 (also know as SCOT), encoded by nuclear gene, is a mitochondrial CoA transferase required for ketone body degradation. It catalyzes the transfer of CoA from succinyl-CoA to acetoacetate, generating acetoacetyl-CoA. OXCT1 is expressed in brain, heart, and skeletal muscle, but not in liver. This antibody specifically recognizes endogenous OXCT1. (21209089)