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OXCT1 Monoclonal antibody
OXCT1 Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat, pig, rabbit
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
1G1B9
Cat no : 67836-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | rat heart tissue, HeLa cells, Jurkat cells, pig brain tissue, rat brain tissue, mouse brain tissue, rabbit brain tissue |
Positive IHC detected in | rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | MCF-7 cells, HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Sample-dependent, check data in validation data gallery |
Product Information
67836-1-Ig targets OXCT1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.
Tested Reactivity | Human, mouse, rat, pig, rabbit |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | OXCT1 fusion protein Ag24792 |
Full Name | 3-oxoacid CoA transferase 1 |
Calculated molecular weight | 520 aa, 56 kDa |
Observed molecular weight | 52-56 kDa |
GenBank accession number | BC009001 |
Gene symbol | OXCT1 |
Gene ID (NCBI) | 5019 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
OXCT1 (also know as SCOT), encoded by nuclear gene, is a mitochondrial CoA transferase required for ketone body degradation. It catalyzes the transfer of CoA from succinyl-CoA to acetoacetate, generating acetoacetyl-CoA. OXCT1 is expressed in brain, heart, and skeletal muscle, but not in liver. This antibody specifically recognizes endogenous OXCT1. (21209089)
Protocols
Product Specific Protocols | |
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WB protocol for OXCT1 antibody 67836-1-Ig | Download protocol |
IHC protocol for OXCT1 antibody 67836-1-Ig | Download protocol |
IF protocol for OXCT1 antibody 67836-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |