ORAI1 Polyclonal antibody

ORAI1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 14443-1-AP

Synonyms

ORAI1, ORAI1 L1, ORAI1 L2, Orail L2, TMEM142A



Tested Applications

Positive WB detected inhuman spleen tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

14443-1-AP targets ORAI1 in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name ORAI calcium release-activated calcium modulator 1
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberNM_032790
Gene Symbol ORAI1
Gene ID (NCBI) 84876
RRIDAB_10639506
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Store-operated calcium channels (SOCs) play essential functions in various physiological processes. Orai1 (also named CRACM1) is a critical molecular component of SOCs. Orai1 protein is a 35-45 kDa plasma membrane protein with 4 transmembrane domains. Human patients with a severe combinaed immunodeficiency (SCID) syndrome possess point mutations in Orai1 gene. This antibody may be used to block the ion currents mediated by Orai1. ORAI1 contains 2 isoforms  produced by alternative initiation(uniprot: Q96D31). Catalog#14443-1-AP only recognises alpha isoform.

Protocols

Product Specific Protocols
WB protocol for ORAI1 antibody 14443-1-APDownload protocol
IHC protocol for ORAI1 antibody 14443-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

PLoS One

Coupling of store-operated calcium entry to vasoconstriction is acid-sensing ion channel 1a dependent in pulmonary but not mesenteric arteries.

Authors - Selina M Garcia