Biotin-conjugated P-cadherin Polyclonal antibody

P-cadherin Polyclonal Antibody for IHC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC

Conjugate

Biotin

Publications

1

Cat no : Biotin-13773

Synonyms

P cadherin, HJMD, CDHP, CDH3, Cadherin-3

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Validation Data Gallery

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  IHC staining of human breast cancer using Biotin-13773

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using Biotin-13773 (P-cadherin antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using Biotin-13773

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using Biotin-13773 (P-cadherin antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

Biotin-13773 targets P-cadherin in IHC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: P-cadherin fusion protein Ag4758
• Full Name: cadherin 3, type 1, P-cadherin (placental)
• Calculated Molecular Weight: 829 aa, 91 kDa
• GenBank Accession Number: BC041846
• Gene Symbol: P-cadherin
• Gene ID (NCBI): 1001
• RRID: AB_2883057
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. P-cadherin (placental cadherin), also known as CDH3 (cadherin 3), is a classical member of the cadherin superfamily which also include E-, N-, R-, and B-cadherins. Aberrant expression of P-cadherin has been reported in many cancers, including breast, gastric, endometrial, ovarian, and prostate cancer.

Publications

Species	Application	Title
mouse	IHC	J Invest Dermatol; Lgr4 Deletion Delays the Hair Cycle and Inhibits the Activation of Hair Follicle Stem Cells.; Authors - Xiaolin Ren