Tested Applications
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
Biotin-13773 targets P-cadherin in IHC applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4758 Product name: Recombinant human CDH3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 24-349 aa of BC041846 Sequence: SEPCRAVFREAEVTLEAGGAEQEPGQALGKVFMGCPGQEPALFSTDNDDFTVRNGETVQERRSLKERNPLKIFPSKRILRRHKRDWVVAPISVPENGKGPFPQRLNQLKSNKDRDTKIFYSITGPGADSPPEGVFAVEKETGWLLLNKPLDREEIAKYELFGHAVSENGASVEDPMNISIIVTDQNDHKPKFTQDTFRGSVLEGVLPGTSVMQMTATDEDDAIYTYNGVVAYSIHSQEPKDPHDLMFTIHRSTGTISVISSGLDREKVPEYTLTIQATDMDGDGSTTTAVAVVEILDANDNAPMFDPQKYEAHVPENAVGHEVQRL Predict reactive species |
| Full Name | cadherin 3, type 1, P-cadherin (placental) |
| Calculated Molecular Weight | 829 aa, 91 kDa |
| GenBank Accession Number | BC041846 |
| Gene Symbol | P-cadherin |
| Gene ID (NCBI) | 1001 |
| RRID | AB_2883057 |
| Conjugate | Biotin |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P22223 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. P-cadherin (placental cadherin), also known as CDH3 (cadherin 3), is a classical member of the cadherin superfamily which also include E-, N-, R-, and B-cadherins. Aberrant expression of P-cadherin has been reported in many cancers, including breast, gastric, endometrial, ovarian, and prostate cancer.
Publications
What published studies show
Biotin-13773 is a biotin-conjugated P-cadherin (CDH3) antibody. It has a total of 1 citation. The cited publication appeared in the Journal of Investigative Dermatology (IF: 7), published in February 2020. The study investigated Lgr4 deletion and its effects on hair cycle progression and hair follicle stem cell activation in mouse models. The antibody was applied via immunohistochemistry (IHC). The research field falls within dermatology and hair follicle biology.
| Species | Application | Title |
|---|---|---|
J Invest Dermatol Lgr4 Deletion Delays the Hair Cycle and Inhibits the Activation of Hair Follicle Stem Cells. |
