|Positive WB detected in||A549 cells, HeLa cells, SH-SY5Y cells|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
13534-1-AP targets P2RX4 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||P2RX4 fusion protein Ag4445|
|Full Name||purinergic receptor P2X, ligand-gated ion channel, 4|
|Calculated molecular weight||388 aa, 43 kDa|
|Observed molecular weight||70-80 kDa, 50-60 kDa|
|GenBank accession number||BC033826|
|Gene ID (NCBI)||5025|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Nucleotides, the structural subunits of the nucleic acids, are also important extracellular signaling molecules. P2 receptors are a family of cell surface receptors that mediate a wide variety of physiologic effects in response to extracellular nucleotides. These receptors fall into two classes: P2X receptors, which are ligand-gated ion channels that mediate calcium and potassium fluxes in response to ATP, and P2Y receptors, which are G protein-coupled receptors (GPCRs). P2RX4 (P2X purinoceptor 4) is a receptor for ATP acting as a ligand gated ion channel. The calculated molecular weight of P2RX4 is 43 kDa, larger apparent molecular weights of 50-80 kDa have been reported, possibly due to post-translational glycosylation (PMID: 24040145, 15262999; 29382907; 12566439).
|Product Specific Protocols|
|WB protocol for P2RX4 antibody 13534-1-AP||Download protocol|
|IHC protocol for P2RX4 antibody 13534-1-AP||Download protocol|
|IF protocol for P2RX4 antibody 13534-1-AP||Download protocol|
|IP protocol for P2RX4 antibody 13534-1-AP||Download protocol|
|FC protocol for P2RX4 antibody 13534-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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