P2RX6 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis of mouse skeletal muscle using 84458-4-RR (same clone as 84458-4-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 84458-4-RR (P2RX6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84458-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84458-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84458-4-RR against Human P2RX6 were performed. The affinity constant is below 1 pM.

P2RX6 Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 84458-4-PBS

Clone No.241620B10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

P2XM, P2X6, P2X purinoceptor 6, P2RXL1, ATP receptor

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Product Information

84458-4-PBS targets P2RX6 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag12331

  Product name: Recombinant human P2RX6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-330 aa of BC033488

  Sequence: MGSPGATTGWGLLDYKTEKYVMTRNWRVGALQRLLQFGIVVYVVGWALLAKKGYQERDLEPQFSIITKLKGVSVTQIKELGNRLWDVADFVKPPQGENVFFLVTNFLVTPAQVQGRCPEHPSVPLANCWVDEDCPEGEGGTHSHGVKTGQCVVFNGTHRTCEIWSWCPVESGVVPSRPLLAQAQNFTLFIKNTVTFSKFNFSKSNALETWDPTYFKHCRYEPQFSPYCPVFRIGDLVAKAGGTFEDLALLGGSVGIRVHWDCDLDTGDSGCWPHYSFQLQEKSYNFRTATHWWEQPGVEARTLLKLYGIRFDILVTGQAGKFGLIPTAVT

• Full Name: purinergic receptor P2X, ligand-gated ion channel, 6
• Calculated Molecular Weight: 431 aa, 48 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC033488
• Gene Symbol: P2RX6
• Gene ID (NCBI): 9127
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O15547
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The P2 receptors have two subfamilies in mammalian cells, P2X and P2Y. P2X family of ligand-gated ion channel receptors have seven members, P2RX1-7, which all constitute non-selective cation channels proposed to be functional under a homo- or heterotrimeric association. P2RX6 is predominantly expressed in skeletal muscle, and regulated by p53. It's reported that ATP might increase renal cell carcinoma cells migration and invasion through P2RX6.(PMID: 31159832, PMID:9242461)