P2RX6 Recombinant antibody

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  WB analysis of mouse skeletal muscle using 84458-4-RR

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 84458-4-RR (P2RX6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84458-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84458-4-RR against Human P2RX6 were performed. The affinity constant is below 1 pM.

P2RX6 Recombinant Antibody for WB, ELISA

Cat No. 84458-4-RR

Clone No.241620B10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

P2XM, P2X6, P2X purinoceptor 6, P2RXL1, ATP receptor

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84458-4-RR targets P2RX6 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: P2RX6 fusion protein Ag12331
• Full Name: purinergic receptor P2X, ligand-gated ion channel, 6
• Calculated Molecular Weight: 431 aa, 48 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC033488
• Gene Symbol: P2RX6
• Gene ID (NCBI): 9127
• RRID: AB_3671983
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O15547
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The P2 receptors have two subfamilies in mammalian cells, P2X and P2Y. P2X family of ligand-gated ion channel receptors have seven members, P2RX1-7, which all constitute non-selective cation channels proposed to be functional under a homo- or heterotrimeric association. P2RX6 is predominantly expressed in skeletal muscle, and regulated by p53. It's reported that ATP might increase renal cell carcinoma cells migration and invasion through P2RX6.(PMID: 31159832, PMID:9242461)