P4HA2 Monoclonal antibody, PBS Only

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  WB analysis of HeLa using 66604-1-Ig (same clone as 66604-1-PBS)

  WB result of P4HA2 antibody (66604-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-P4HA2 transfected HeLa cells. This data was developed using the same antibody clone with 66604-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 66604-1-Ig (same clone as 66604-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66604-1-Ig (P4HA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66604-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 66604-1-Ig (same clone as 66604-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66604-1-Ig (P4HA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66604-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66604-1-Ig (same clone as 66604-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66604-1-Ig (P4HA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66604-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66604-1-Ig (same clone as 66604-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66604-1-Ig (P4HA2 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66604-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66604-1-Ig (same clone as 66604-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66604-1-Ig (P4HA2 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66604-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

P4HA2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 66604-1-PBS

Clone No.3A9A4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

4 PH alpha 2, 4-PH alpha-2, EC:1.14.11.2, Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-2, Prolyl 4-hydroxylase subunit alpha-2

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Product Information

66604-1-PBS targets P4HA2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4714

  Product name: Recombinant human P4HA2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 184-533 aa of BC035813

  Sequence: HTVLWMEQVLKQLDAGEEATTTKSQVLDYLSYAVFQLGDLHRALELTRRLLSLDPSHERAGGNLRYFEQLLEEEREKTLTNQTEAELATPEGIYERPVDYLPERDVYESLCRGEGVKLTPRRQKRLFCRYHHGNRAPQLLIAPFKEEDEWDSPHIVRYYDVMSDEEIERIKEIAKPKLARATVRDPKTGVLTVASYRVSKSSWLEEDDDPVVARVNRRMQHITGLTVKTAELLQVANYGVGGQYEPHFDFSRRPFDSGLKTEGNRLATFLNYMSDVEAGGATVFPDLGAAIWPKKGTAVFWYNLLRSGEGDYRTRHAACPVLVGCKWVSNKWFHERGQEFLRPCGSTEVD

• Full Name: prolyl 4-hydroxylase, alpha polypeptide II
• Calculated Molecular Weight: 533 aa, 61 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: BC035813
• Gene Symbol: P4HA2
• Gene ID (NCBI): 8974
• RRID: AB_2881964
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O15460
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Prolyl 4-hydroxylase catalyzes the posttranslational formation of 4-hydroxyproline in collagens. The vertebrate enzyme is a tetramer of 2 alpha subunits and 2 beta subunits. P4HA2 is one of at least 2 alpha subunit isoforms. It catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. This pprotein has 2 isoforms produced by alternative splicing.