P4HA3 Recombinant monoclonal antibody

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  WB analysis using 85374-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85374-1-RR (P4HA3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 85374-1-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 85374-1-RR (P4HA3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 85374-1-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 85374-1-RR (P4HA3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 85374-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using P4HA3 antibody (85374-1-RR, Clone: 242750C3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity of 85374-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85374-1-RR against Human P4HA3 were performed. The affinity constant is below 1 pM.

P4HA3 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 85374-1-RR

Clone No.242750C3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

4-PH alpha-3, EC:1.14.11.2, Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-3, Prolyl 4-hydroxylase subunit alpha-3, UNQ711/PRO1374

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85374-1-RR targets P4HA3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag19716

  Product name: Recombinant human P4HA3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 28-256 aa of BC126170

  Sequence: GDTFSALTSVARALAPERRLLGLLRRYLRGEEARLRDLTRFYDKVLSLHEDSTTPVANPLLAFTLIKRLQSDWRNVVHSLEASENIRALKDGYEKVEQDLPAFEDLEGAARALMRLQDVYMLNVKGLARGVFQRVTGSAITDLYSPKRLFSLTGDDCFQVGKVAYDMGDYYHAIPWLEEAVSLFRGSYGEWKTEDEASLEDALDHLAFAYFRAGNVSCALSLSREFLLY

• Full Name: prolyl 4-hydroxylase, alpha polypeptide III
• Calculated Molecular Weight: 544 aa, 61 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: BC126170
• Gene Symbol: P4HA3
• Gene ID (NCBI): 283208
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q7Z4N8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Collagen prolyl 4-hydroxylases catalyze the hydroxylation of proline in x-pro-gly triplets in collagens and in proteins with collagen-like sequences. Collagen prolyl 4-hydroxylases are tetramers of 2 alpha subunits, such as P4HA3, and 2 beta subunits(P4HB). P4HA3 has 3 isoforms produced by alternative splicing with the MW of 61 kDa, 29 kDa and 67 kDa.