CoraLite® Plus 488-conjugated P4HA3 Recombinant monoclonal antibody

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• CoraLite® Plus 488-conjugated P4HA3 Recombinant monoclonal antibody (CL488-85374)

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  IF Staining of A549 using CL488-85374

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite® Plus 488 P4HA3 antibody (CL488-85374, Clone: 242750C3 ) at dilution of 1:200.

P4HA3 Uni-rAb^® Recombinant Antibody for IF/ICC

Cat No. CL488-85374

Clone No.242750C3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

4-PH alpha-3, EC:1.14.11.2, Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-3, Prolyl 4-hydroxylase subunit alpha-3, UNQ711/PRO1374

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Tested Applications

• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-85374 targets P4HA3 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag19716

  Product name: Recombinant human P4HA3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 28-256 aa of BC126170

  Sequence: GDTFSALTSVARALAPERRLLGLLRRYLRGEEARLRDLTRFYDKVLSLHEDSTTPVANPLLAFTLIKRLQSDWRNVVHSLEASENIRALKDGYEKVEQDLPAFEDLEGAARALMRLQDVYMLNVKGLARGVFQRVTGSAITDLYSPKRLFSLTGDDCFQVGKVAYDMGDYYHAIPWLEEAVSLFRGSYGEWKTEDEASLEDALDHLAFAYFRAGNVSCALSLSREFLLY

• Full Name: prolyl 4-hydroxylase, alpha polypeptide III
• Calculated Molecular Weight: 544 aa, 61 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: BC126170
• Gene Symbol: P4HA3
• Gene ID (NCBI): 283208
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Excitation Laser: Blue laser (488 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q7Z4N8
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Collagen prolyl 4-hydroxylases catalyze the hydroxylation of proline in x-pro-gly triplets in collagens and in proteins with collagen-like sequences. Collagen prolyl 4-hydroxylases are tetramers of 2 alpha subunits, such as P4HA3, and 2 beta subunits(P4HB). P4HA3 has 3 isoforms produced by alternative splicing with the MW of 61 kDa, 29 kDa and 67 kDa.