CoraLite® Plus 488-conjugated P53 Recombinant antibody
P53 Recombinant Antibody for FC (Intra), IF
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse, rat
Applications
IF, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2H13
Cat no : CL488-80077
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | HepG2 cells |
Positive FC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
CL488-80077 targets P53 in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.
Tested Reactivity | Human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Peptide |
Full Name | tumor protein p53 |
Calculated molecular weight | 44 kDa |
Observed molecular weight | 53 kDa |
GenBank accession number | BC003596 |
Gene symbol | TP53 |
Gene ID (NCBI) | 7157 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TP53, also known as P53 and NY-CO-13, belongs to the p53 family and has 9 isoforms. In SDS-Page, the observed molecular weight is about 53 kDa. TP53 acts as a tumor suppressor in many tumor types, including growth arrest or apoptosis depending on the physiological circumstances and cell types. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. TP53 Localizes in the nucleus in most cells but found in the cytoplasm in some cells. (PMID: 26166714; PMID: 25225161)
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 P53 antibody CL488-80077 | Download protocol |
FC protocol for CL Plus 488 P53 antibody CL488-80077 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |