Tested Applications
| Positive WB detected in | HEK-293T cells, HEK-293 cells, rat spleen tissue, HL-60 cells, HeLa cells, Jurkat cells, MCF-7 cells, PC-3 cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15348-1-AP targets PA2G4 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7587 Product name: Recombinant human PA2G4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-394 aa of BC001951 Sequence: MSGEDEQQEQTIAEDLVVTKYKMGGDIANRVLRSLVEASSSGVSVLSLCEKGDAMIMEETGKIFKKEKEMKKGIAFPTSISVNNCVCHFSPLKSDQDYILKEGDLVKIDLGVHVDGFIANVAHTFVVDVAQGTQVTGRKADVIKAAHLCAEAALRLVKPGNQNTQVTEAWNKVAHSFNCTPIEGMLSHQLKQHVIDGEKTIIQNPTDQQKKDHEKAEFEVHEVYAVDVLVSSGEGKAKDAGQRTTIYKRDPSKQYGLKMKTSRAFFSEVERRFDAMPFTLRAFEDEKKARMGVVECAKHELLQPFNVLYEKEGEFVAQFKFTVLLMPNGPMRITSGPFEPDLYKSEMEVQDAELKALLQSSASRKTQKKKKKKASKTAENATSGETLEENEAGD Predict reactive species |
| Full Name | proliferation-associated 2G4, 38kDa |
| Calculated Molecular Weight | 44 kDa |
| Observed Molecular Weight | 42 kDa, 48 kDa |
| GenBank Accession Number | BC001951 |
| Gene Symbol | PA2G4 |
| Gene ID (NCBI) | 5036 |
| RRID | AB_2156602 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UQ80 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PA2G4 (EBP1), first identified as an ErbB3 binding protein, is a 38 kDa protein that is widely expressed in cultured cells and tissues. The presence of several functional domains such as a nuclear localization sequence (NLS), LxxLL and LxCxE suggests the involvement of Ebp1 in cell signaling pathways and gene transcription regulation. This antibody is a rabbit polyclonal antibody raised against full length PA2G4 of human origin.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PA2G4 antibody 15348-1-AP | Download protocol |
| IHC protocol for PA2G4 antibody 15348-1-AP | Download protocol |
| IP protocol for PA2G4 antibody 15348-1-AP | Download protocol |
| WB protocol for PA2G4 antibody 15348-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PA2G4 Polyclonal antibody (Catalog No. 15348-1-AP) has 10 total citations. These appear in journals including Nature Communications, Molecular Cell, Advanced Science, Nature Cardiovascular Research, Cell Biosciences, FASEB Journal, Journal of Dental Sciences, Applied Immunohistochemistry & Molecular Morphology, and Methods in Enzymology. The research fields span oncology (AML, prostate, hepatocellular, nasopharyngeal, and head and neck cancers), RNA biology and ribosome profiling, cardiovascular regeneration, reproductive biology (spermatogenesis, ovarian insufficiency), and immune receptor signaling. Applications include WB, IF, IHC, CoIP, IP, and RIP.
| Species | Application | Title |
|---|---|---|
Nat Commun Orthogonal proteogenomic analysis identifies the druggable PA2G4-MYC axis in 3q26 AML | ||
Mol Cell Cell-surface RNA forms ternary complex with RNA-binding proteins and heparan sulfate to recruit immune receptors. | ||
Adv Sci (Weinh) Primate-Specific DAZ Regulates Translation of Cell Proliferation-Related mRNAs and is Essential for Maintenance of Spermatogonia | ||
Nat Cardiovasc Res The highly conserved PIWI-interacting RNA CRAPIR antagonizes PA2G4-mediated NF110-NF45 disassembly to promote heart regeneration in mice | ||
Cell Biosci PA2G4 in CAFs promotes biochemical recurrence of prostate cancer via H3K18la.
| ||
Cell Biosci PA2G4 promotes the metastasis of hepatocellular carcinoma by stabilizing FYN mRNA in a YTHDF2-dependent manner.
|
