Tested Applications
| Positive WB detected in | HeLa cells, LNCaP cells, MCF-7 cells, T-47D cells, NCCIT cells |
| Positive IHC detected in | mouse testis tissue, mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66809-1-Ig targets PABPC1,PABP in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, hamster |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27860 Product name: Recombinant human PABPC1,PABP protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 287-636 aa of BC015958 Sequence: RITRYQGVNLYVKNLDDGIDDERLRKEFSPFGTITSAKVMMEGGRSKGFGFVCFSSPEEATKAVTEMNGRIVATKPLYVALAQRKEERQAHLTNQYMQRMASVRAVPNPVINPYQPAPPSGYFMAAIPQTQNRAAYYPPSQIAQLRPSPRWTAQGARPHPFQNMPGAIRPAAPRPPFSTMRPASSQVPRVMSTQRVANTSTQTMGPRPAAAAAAATPAVRTVPQYKYAAGVRNPQQHLNAQPQVTMQQPAVHVQGQEPLTASMLASAPPQEQKQMLGERLFPLIQAMHPTLAGKITGMLLEIDNSELLHMLESPESLRSKVDEAVAVLQAHQAKEAAQKAVNSATGVPTV Predict reactive species |
| Full Name | poly(A) binding protein, cytoplasmic 1 |
| Calculated Molecular Weight | 71 kDa |
| Observed Molecular Weight | 71 kDa |
| GenBank Accession Number | BC015958 |
| Gene Symbol | PABPC1 |
| Gene ID (NCBI) | 26986 |
| RRID | AB_2882152 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P11940 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation [PMID: 21989405]. Polyadenylate-binding protein 1 (PABPC1) is a cytoplasmic-nuclear shuttling protein important for protein translation initiation, and both RNA processing and stability. In the cytoplasm, PABPC1 binds to the 3' poly(A) tail of eukaryotic mRNAs through its RNA-recognition motifs (RRM) and interacts with the N-terminus of eIF4G, part of the eIF4F complex associated with the 5' cap structure [PMID:20009508, 17381337].
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PABPC1,PABP antibody 66809-1-Ig | Download protocol |
| IHC protocol for PABPC1,PABP antibody 66809-1-Ig | Download protocol |
| WB protocol for PABPC1,PABP antibody 66809-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Anti-PABPC1 antibody (Cat# 66809-1-Ig) has 13 citations published in journals including Nature Communications, Molecular Cell, Advanced Science, Cell Death & Disease, Cancer Letters, Acta Pharmacologica Sinica, Genes & Development, Journal of Hypertension, European Journal of Medical Research, RNA, and Future Oncology. Research fields span mRNA translation regulation, stress granule assembly, phase separation, gastric/pancreatic/renal cancers, viral cap-independent translation, and preeclampsia.
| Species | Application | Title |
|---|---|---|
Nat Commun An engineered linear cap-independent mRNA vaccine with intrinsic adjuvanticity induces potent anti-tumor immunity in mice. | ||
Mol Cell Longitudinal monitoring of cytoplasmic RBP-RNA interactions and transcriptome in living cells by engineered protein nanocages. | ||
Adv Sci (Weinh) Helicobacter Pylori-Enhanced hnRNPA2B1 Coordinates with PABPC1 to Promote Non-m6A Translation and Gastric Cancer Progression | ||
Adv Sci (Weinh) DDX3 Regulates the Cap-Independent Translation of the Japanese Encephalitis Virus via Its Interactions with PABP1 and the Untranslated Regions of the Viral Genome
| ||
Cell Death Dis PABPC1-induced stabilization of PGK1 mRNA reduces apoptosis and sunitinib sensitivity in renal cell carcinoma by suppressing endoplasmic reticulum stress.
| ||
Cancer Lett Hypoxia-driven phase separation of the PABP1/eIF4B complex forms stress granules and activates ChaC2 translation to promote polyunsaturated lipids-supported peritoneal metastasis in gastric cancer. |
