Various lysates were subjected to SDS PAGE followed by western blot with 66809-1-Ig (PABPC1,PABP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 66809-1-Ig (PABPC1,PABP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 66809-1-Ig
WB result of PABPC1,PABP antibody (66809-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PABPC1,PABP transfected HeLa cells.
WB result of PABPC1,PABP antibody (66809-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PABPC1,PABP transfected HeLa cells.
WB analysis of LNCaP using 66809-1-Ig
LNCaP cells were subjected to SDS PAGE followed by western blot with 66809-1-Ig (PABPC1,PABP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
LNCaP cells were subjected to SDS PAGE followed by western blot with 66809-1-Ig (PABPC1,PABP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC staining of mouse testis using 66809-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66809-1-Ig (PABPC1,PABP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66809-1-Ig (PABPC1,PABP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HEK-293 using 66809-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 66809-1-Ig (PABPC1,PABP antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 66809-1-Ig (PABPC1,PABP antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
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HeLa cells, LNCaP cells, MCF-7 cells, T-47D cells, NCCIT cells
Positive IHC detected in
mouse testis tissue, mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HEK-293 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:2000-1:10000
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
The poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation [PMID:
21989405]. Polyadenylate-binding protein 1 (PABPC1) is a cytoplasmic-nuclear shuttling protein important for protein translation initiation, and both RNA processing and stability. In the cytoplasm, PABPC1 binds to the 3' poly(A) tail of eukaryotic mRNAs through its RNA-recognition motifs (RRM) and interacts with the N-terminus of eIF4G, part of the eIF4F complex associated with the 5' cap structure [PMID:20009508, 17381337].
Pyridostigmine ameliorates preeclamptic features in pregnant rats by inhibiting tumour necrosis factor-α synthetsis and antagonizing tumour necrosis factor-α-related effects.
Various lysates were subjected to SDS PAGE followed by western blot with 66809-1-Ig (PABPC1,PABP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 66809-1-Ig
WB result of PABPC1,PABP antibody (66809-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PABPC1,PABP transfected HeLa cells.
WB analysis of LNCaP using 66809-1-Ig
LNCaP cells were subjected to SDS PAGE followed by western blot with 66809-1-Ig (PABPC1,PABP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of mouse testis using 66809-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66809-1-Ig (PABPC1,PABP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 66809-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66809-1-Ig (PABPC1,PABP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse cerebellum using 66809-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66809-1-Ig (PABPC1,PABP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse cerebellum using 66809-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66809-1-Ig (PABPC1,PABP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse cerebellum using 66809-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66809-1-Ig (PABPC1,PABP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse cerebellum using 66809-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66809-1-Ig (PABPC1,PABP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of HEK-293 using 66809-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 66809-1-Ig (PABPC1,PABP antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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