PABPC1,PABP Monoclonal antibody

PABPC1,PABP Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

3D8B5

Cat no : 66809-1-Ig

Synonyms

PAB1, PABP, PABP 1, PABP1, PABPC1, PABPC2, PABPL1, Poly(A) binding protein 1



Tested Applications

Positive WB detected inHeLa cells, LNCaP cells, MCF-7 cells, T47D cells
Positive IHC detected inmouse testis tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66809-1-Ig targets PABPC1,PABP in WB, IHC, IF,ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PABPC1,PABP fusion protein Ag27860
Full Name poly(A) binding protein, cytoplasmic 1
Calculated molecular weight 71 kDa
Observed molecular weight 71 kDa
GenBank accession numberBC015958
Gene symbol PABPC1
Gene ID (NCBI) 26986
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation [PMID:21989405]. Polyadenylate-binding protein 1 (PABPC1) is a cytoplasmic-nuclear shuttling protein important for protein translation initiation, and both RNA processing and stability. In the cytoplasm, PABPC1 binds to the 3' poly(A) tail of eukaryotic mRNAs through its RNA-recognition motifs (RRM) and interacts with the N-terminus of eIF4G, part of the eIF4F complex associated with the 5' cap structure [PMID:20009508, 17381337].

Protocols

Product Specific Protocols
WB protocol for PABPC1,PABP antibody 66809-1-IgDownload protocol
IHC protocol for PABPC1,PABP antibody 66809-1-IgDownload protocol
IF protocol for PABPC1,PABP antibody 66809-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols