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PABPC1,PABP Monoclonal antibody
PABPC1,PABP Monoclonal Antibody for IF, IHC, WB, ELISA
Cat no : 66809-1-Ig
Validation Data Gallery
|Positive WB detected in||HeLa cells, LNCaP cells, MCF-7 cells, T47D cells|
|Positive IHC detected in||mouse testis tissue, mouse cerebellum tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HEK-293 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66809-1-Ig targets PABPC1,PABP in WB, IHC, IF, ELISA applications and shows reactivity with Human, rat samples.
|Tested Reactivity||Human, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||PABPC1,PABP fusion protein Ag27860|
|Full Name||poly(A) binding protein, cytoplasmic 1|
|Calculated molecular weight||71 kDa|
|Observed molecular weight||71 kDa|
|GenBank accession number||BC015958|
|Gene ID (NCBI)||26986|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation [PMID: 21989405]. Polyadenylate-binding protein 1 (PABPC1) is a cytoplasmic-nuclear shuttling protein important for protein translation initiation, and both RNA processing and stability. In the cytoplasm, PABPC1 binds to the 3' poly(A) tail of eukaryotic mRNAs through its RNA-recognition motifs (RRM) and interacts with the N-terminus of eIF4G, part of the eIF4F complex associated with the 5' cap structure [PMID:20009508, 17381337].
The prognostic impacts of PABPC1 expression on gastric cancer patients.
Pyridostigmine ameliorates preeclamptic features in pregnant rats by inhibiting tumour necrosis factor-α synthetsis and antagonizing tumour necrosis factor-α-related effects.