CoraLite®594-conjugated PABPC1,PABP Monoclonal antibody
PABPC1,PABP Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG1
Reactivity
Human, rat
Applications
IF
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
3D8B5
Cat no : CL594-66809
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | HEK-293 cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
CL594-66809 targets PABPC1,PABP in IF applications and shows reactivity with Human, rat samples.
Tested Reactivity | Human, rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | PABPC1,PABP fusion protein Ag27860 |
Full Name | poly(A) binding protein, cytoplasmic 1 |
Calculated molecular weight | 71 kDa |
Observed molecular weight | 71 kDa |
GenBank accession number | BC015958 |
Gene symbol | PABPC1 |
Gene ID (NCBI) | 26986 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation [PMID:21989405]. Polyadenylate-binding protein 1 (PABPC1) is a cytoplasmic-nuclear shuttling protein important for protein translation initiation, and both RNA processing and stability. In the cytoplasm, PABPC1 binds to the 3' poly(A) tail of eukaryotic mRNAs through its RNA-recognition motifs (RRM) and interacts with the N-terminus of eIF4G, part of the eIF4F complex associated with the 5' cap structure [PMID:20009508, 17381337].
Protocols
Product Specific Protocols | |
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IF protocol for CL594 PABPC1,PABP antibody CL594-66809 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |