CoraLite®594-conjugated PABPC1,PABP Monoclonal antibody

PABPC1,PABP Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat and More (1)

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

Publications

1

CloneNo.

3D8B5

Cat no : CL594-66809

Synonyms

PAB1, PABP, PABP 1, PABP1, PABPC1, PABPC2, PABPL1, Poly(A) binding protein 1

---

Validation Data Gallery

• 

  IF Staining of HEK-293 using CL594-66809

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite®594-conjugated PABPC1,PABP antibody (CL594-66809, Clone: 3D8B5 ) at dilution of 1:100.

---

Tested Applications

• Positive IF detected in: HEK-293 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

CL594-66809 targets PABPC1,PABP in IF applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PABPC1,PABP fusion protein Ag27860
• Full Name: poly(A) binding protein, cytoplasmic 1
• Calculated Molecular Weight: 71 kDa
• Observed Molecular Weight: 71 kDa
• GenBank Accession Number: BC015958
• Gene Symbol: PABPC1
• Gene ID (NCBI): 26986
• RRID: AB_2883628
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation [PMID:21989405]. Polyadenylate-binding protein 1 (PABPC1) is a cytoplasmic-nuclear shuttling protein important for protein translation initiation, and both RNA processing and stability. In the cytoplasm, PABPC1 binds to the 3' poly(A) tail of eukaryotic mRNAs through its RNA-recognition motifs (RRM) and interacts with the N-terminus of eIF4G, part of the eIF4F complex associated with the 5' cap structure [PMID:20009508, 17381337].

Publications

Species	Application	Title
human	IF	J Biol Chem; A complex with poly(A) binding protein and EWS facilitates the transcriptional function of oncogenic ETS transcription factors in prostate cells; Authors - Benjamin M Greulich