Tested Applications
| Positive IHC detected in | human endometrial cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 2 publications below |
Product Information
18187-1-AP targets PAEP/Glycodelin in IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12902 Product name: Recombinant human PAEP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 54-180 aa of BC069562 Sequence: KAPLRVHITSLLPTPEDNLEIVLHRWENNSCVEKKVLGEKTENPKKFKINYTVANEATLLDTDYDNFLFLCLQDTTTPIQSMMCQYLARVLVEDDEIMQGFIRAFRPLPRHLWYLLDLKQMEEPCRF Predict reactive species |
| Full Name | progestagen-associated endometrial protein |
| Calculated Molecular Weight | 180 aa, 21 kDa |
| Observed Molecular Weight | 24-28 kDa |
| GenBank Accession Number | BC069562 |
| Gene Symbol | PAEP/Glycodelin |
| Gene ID (NCBI) | 5047 |
| RRID | AB_2918059 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P09466 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Glycodelin (also known as Progestagen Associated Endometrial Protein, PAEP, and Placental Protein 14, PP14) is a secreted human lipocalin-family protein mainly expressed in well-differentiated epithelial cells in human reproductive tissues, like endometrium and seminal vesicles. While different cells produce differentially glycosylated isoforms of Glycodelin: decidualized endometrium-derived glycodelin-A (GdA), seminal vesicle-derived glycodelin-S (GdS), glycodelin-F (isolated from follicular fluid), and glycodelin-C (isolated from cumulus cells). Glycodelin has been involved in cell differentiation and tumor growth.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PAEP/Glycodelin antibody 18187-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Clin Transl Med Single-cell transcriptomes reveal heterogeneity of high-grade serous ovarian carcinoma. | ||
Front Immunol Single-cell sequencing reveals effects of chemotherapy on the immune landscape and TCR/BCR clonal expansion in a relapsed ovarian cancer patient |
