PAK1 Polyclonal antibody, PBS Only

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  WB analysis using 51137-1-AP (same clone as 51137-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 51137-1-AP (PAK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51137-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 51137-1-AP (same clone as 51137-1-PBS)

  WB result of PAK1 antibody (51137-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PAK1 transfected HEK-293 cells. This data was developed using the same antibody clone with 51137-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 51137-1-AP (same clone as 51137-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 51137-1-AP (PAK1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51137-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 51137-1-AP (same clone as 51137-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 51137-1-AP (PAK1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51137-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 51137-1-AP (same clone as 51137-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 51137-1-AP (PAK1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 51137-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 51137-1-AP (same clone as 51137-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 51137-1-AP (PAK1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 51137-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 51137-1-AP (same clone as 51137-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PAK1 antibody (51137-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 51137-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

PAK1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 51137-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

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Product Information

51137-1-PBS targets PAK1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: p21 protein (Cdc42/Rac)-activated kinase 1
• Calculated Molecular Weight: 553 aa, 62 kDa
• Observed Molecular Weight: 61-65 kDa
• GenBank Accession Number: BC109299
• Gene Symbol: PAK1
• Gene ID (NCBI): 5058
• RRID: AB_2283523
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13153
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The p21-Activated kinase 1 (PAK1), a member of serine-threonine kinases family, was initially identified as an interactor of the Rho GTPases RAC1 and CDC42, which affect a wide range of processes associated with cell motility, survival, metabolism, cell cycle, proliferation, transformation, stress, inflammation, and gene expression(PMID: 32863957). PAK1 is over-expressed in various malignancies such as ovarian, breast, lung, and bladder cancers, and is closely associated with tumor invasion.