PAMR1 Polyclonal antibody

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  WB analysis of mouse skeletal muscle using 55310-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 55310-1-AP (PAMR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human skeletal muscle using 55310-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55310-1-AP (PAMR1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 55310-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55310-1-AP (PAMR1 antibody) at dilution of 1:50 (under 40x lens).

PAMR1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 55310-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, CoIP, ELISA

Synonyms

DKFZP586H2123, FP938, Inactive serine protease PAMR1, PAMR1, RAMP, UNQ699/PRO1344

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue
• Positive IHC detected in: human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 3 publications below
• CoIP: See 1 publications below

Product Information

55310-1-AP targets PAMR1 in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: regeneration associated muscle protease
• Calculated Molecular Weight: 82 kDa
• Observed Molecular Weight: 78-82 kDa, 67-70 kDa
• GenBank Accession Number: NM_015430
• Gene Symbol: PAMR1
• Gene ID (NCBI): 25891
• RRID: AB_11232034
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6UXH9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PAMR1,also named as RAMP, belongs to the peptidase S1 family. It may play a role in regeneration of skeletal muscle. This antibody is specific to PAMR1.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IHC	Elife; The subiculum is a patchwork of discrete subregions.; Authors - Mark S Cembrowski
human	WB, IHC	Front Oncol; High Expression of PAMR1 Predicts Favorable Prognosis and Inhibits Proliferation, Invasion, and Migration in Cervical Cancer.; Authors - Rui Yang
human,mouse	WB	J Ethnopharmacol; Qizhu Anticancer Prescription enhances immunosurveillance of liver cancer cells by regulating p21-dependent secretory phenotypes; Authors - Rui Hu
human	WB,IHC,CoIP	Funct Integr Genomics; NEDD4 facilitates the progression of endometrial carcinoma by enhancing PAMR1 protein degradation through ubiquitination; Authors - Hongbo Guo