Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55310-1-AP targets PAMR1 in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | regeneration associated muscle protease |
| Calculated Molecular Weight | 82 kDa |
| Observed Molecular Weight | 78-82 kDa, 67-70 kDa |
| GenBank Accession Number | NM_015430 |
| Gene Symbol | PAMR1 |
| Gene ID (NCBI) | 25891 |
| RRID | AB_11232034 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6UXH9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PAMR1,also named as RAMP, belongs to the peptidase S1 family. It may play a role in regeneration of skeletal muscle. This antibody is specific to PAMR1.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PAMR1 antibody 55310-1-AP | Download protocol |
| WB protocol for PAMR1 antibody 55310-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-PAMR1 antibody (Cat# 55310-1-AP) has 4 total citations across journals including J Ethnopharmacol, Funct Integr Genomics, Front Oncol, and eLife. Research fields span hepatocellular carcinoma immunosurveillance, endometrial carcinoma progression via ubiquitination, cervical cancer prognosis, and mouse subiculum subregion mapping. Applications include WB, IHC, and CoIP in human and mouse samples.
| Species | Application | Title |
|---|---|---|
J Ethnopharmacol Qizhu Anticancer Prescription enhances immunosurveillance of liver cancer cells by regulating p21-dependent secretory phenotypes | ||
Funct Integr Genomics NEDD4 facilitates the progression of endometrial carcinoma by enhancing PAMR1 protein degradation through ubiquitination | ||
Front Oncol High Expression of PAMR1 Predicts Favorable Prognosis and Inhibits Proliferation, Invasion, and Migration in Cervical Cancer. | ||
