Anti-Human PANX2 Rabbit Recombinant Antibody

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• Anti-Human PANX2 Rabbit Recombinant Antibody (98696-1-RR)

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  IHC staining of Human brain cancer（Astrocitoma） using 98696-1-RR

  Immunohistochemical analysis of paraffin-embedded Human brain cancer(Astrocitoma) slide using 98696-1-RR (PANX2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of Human brain cancer（Astrocitoma） using 98696-1-RR

  Immunohistochemical analysis of paraffin-embedded Human brain cancer(Astrocitoma) slide using 98696-1-RR (PANX2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of Caco-2 using 98696-1-RR

  1x10^6 Caco-2 cells were surface stained with 0.25 ug Anti-Human PANX2 Rabbit RecAb (98696-2-RR, Clone: 251544A12) or 0.25 ug Isotype Control, and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  FC experiment of Caco-2 using 98696-1-RR

  1x10^6 Caco-2 cells were surface stained with 0.25 ug Anti-Human PANX2 Rabbit RecAb (98696-2-RR, Clone: 251544A12) (red) or 0.25 ug Isotype Control (blue), and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

PANX2 Uni-rAb^® Recombinant Antibody for IHC, FC

Cat No. 98696-1-RR

Clone No.251544B5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, FC

Synonyms

hPANX2, pannexin 2, PX2

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Tested Applications

• Positive IHC detected in: Human brain cancer (Astrocitoma); Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: Caco-2 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98696-1-RR targets PANX2 in IHC, FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: pannexin 2
• Calculated Molecular Weight: 677 aa, 74 kDa
• GenBank Accession Number: BC101023
• Gene Symbol: PANX2
• Gene ID (NCBI): 56666
• RRID: AB_3746281
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96RD6
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Pannexins are glycoproteins that form functional single-membrane channels, which mediate ATP release in response to a variety of physiological and pathological stimuli (PMID: 33913372). The pannexin family consists of three members, namely PANX1, PANX2, and PANX3 (PMID: 22305965). PANX2 was initially predicted to be expressed selectively in the central nervous system, but was later shown to have broad expression across multiple tissues, including skin, skeletal muscle, eye, and colon (PMID: 15028292; 25505382; 25773474). PANX2 is involved in pathophysiological processes of neurological and cancer diseases (PMID: 19749789; 33913372; 39389335).