Tested Applications
| Positive WB detected in | HeLa cells, U-251 cells, U-87 MG cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
34287-1-AP targets PAPOLA in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag41259 Product name: Recombinant human PAPOLA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 560-745 aa of BC036014 Sequence: APAVTAASVTNIQATEVSVPQVNSSESSGGTSSESIPQTATQPAISPPPKPTVSRVVSSTRLVNPPPRSSGNAATSGNAATKIPTPIVGVKRTSSPHKEESPKKTKTEEDETSEDANCLALSGHDKTEAKEQLDTETSTTQSETIQTAASLLASQKTSSTDLSDIPALPANPIPVIKNSIKLRLNR Predict reactive species |
| Full Name | poly(A) polymerase alpha |
| Calculated Molecular Weight | 82 kDa |
| Observed Molecular Weight | 90 kDa |
| GenBank Accession Number | BC036014 |
| Gene Symbol | PAPOLA |
| Gene ID (NCBI) | 10914 |
| RRID | AB_3743186 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | P51003 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Poly(A) polymerase α (PAPOLA) is the most-studied mammalian poly(A) polymerase. Silencing PAPOLA in MCF-7 and MDA-MB-231 breast cancer cells reduced proliferation and anchorage-independent growth by decreasing steady-state cyclin D1 (CCND1) mRNA and protein levels. PAPOLA is a possible candidate target for the control of tumor growth that is mostly relevant to triple-negative tumors, a group characterized by PAPOLA overexpression and lack of alternative targeted therapies (PMID: 33712453). PAPOLA contains various forms of modifications such as acetylation, phosphorylation, and poly-sulfurization.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PAPOLA antibody 34287-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
